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EC number: 201-297-1 | CAS number: 80-62-6
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 987
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only 4 strains of Salmonella typhimurium were tested
- Principles of method if other than guideline:
- Only 4 strains of Salmonella typhimurium were tested
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl methacrylate
- EC Number:
- 201-297-1
- EC Name:
- Methyl methacrylate
- Cas Number:
- 80-62-6
- Molecular formula:
- C5H8O2
- IUPAC Name:
- methyl 2-methylprop-2-enoate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Supplied by Rohm and Haas; 99+% pure
Method
- Target gene:
- his+
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA97, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- AROCLOR 1254 induced rat and Syrian hamster liver S9 mix.
- Test concentrations with justification for top dose:
- 33 - 6666 ug/plate
Test concentrations: 0, 33, 100, 1000, 3333, 6666 µg/plate
At 6666 µg/plate slight cytotoxicity was observed - Vehicle / solvent:
- Not specified: vehicles used included water, DMSO, ethanol or acetone
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: without S9: 4-nitro-o-phenylenediamine (TA98). With S9: 2-aminoanthracene (all strains)
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration: triplicate
- Number of independent experiments: two
METHOD OF TREATMENT/ EXPOSURE:
- Solvent: DMSO
- Cell density at seeding (if applicable):
- After addition of soft agar, the contents of each tube was poured onto minimal medium
TREATMENT AND HARVEST SCHEDULE:
- Preincubation period, if applicable: 20 minutes, 37 °C
- Exposure duration/duration of treatment: 48 hours, 37 °C - Evaluation criteria:
- An individual trial was judged (+) mutagenic if a dose related increase over the corresponding solvent control was seen, and it was judged weakly mutagenic (+W) if a low dose response was seen. A trial was considered questionable (?) if a dose related increase was judged insufficiently high to justify a call of "+W" if only a single dose was elevated over control or if a non-dose related increase was seen. A chemical was judged weakly mutagenic "+W' or mutagenic "+" if it produced a reproducible, dose related increase in his+ revertants over the corresponding solvent controls in replicate trials.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Mutagenicity of Methyl methacrylate in Salmonella Typhimurium |
||||
|
|
Revertants per plate (a,b) |
||
Strain |
Dose (µg/plate) |
-S9 |
+S9 (rat) |
+S9 (hamster) |
TA100 |
0 |
140 +/- 6.7 |
141 +/- 8.0 |
131 +/- 4.7 |
|
33 |
118 +/- 5.6 |
- |
- |
|
100 |
122 +/- 21.1 |
157 +/- 10.2 |
143 +/- 9.9 |
|
333 |
126 +/- 10.5 |
143 +/- 7.1 |
145 +/- 4.1 |
|
1000 |
136 +/- 5.0 |
138 +/- 11.6 |
130 +/- 8.5 |
|
3333 |
129 +/- 8.8 |
121 +/- 10.7 |
116 +/- 6.2 |
|
6666 |
- |
(c) 48+/- 16.3 |
(c) 65+/- 3.5 |
|
|
|
|
|
TA1535 |
0 |
26 +/- 4.4 |
9 +/- 1.5 |
6 +/- 0.6 |
|
33 |
22 +/- 2.2 |
- |
- |
|
100 |
18 +/- 1.7 |
6 +/- 1.2 |
143 +/- 9.9 |
|
333 |
17+/- 2.5 |
8 +/- 2.1 |
145 +/- 4.1 |
|
1000 |
16 +/- 2.3 |
7 +/- 1.0 |
130 +/- 8.5 |
|
3333 |
16 +/- 2.9 |
7 +/- 1.2 |
116 +/- 6.2 |
|
6666 |
- |
(c) 4+/- 0.9 |
(c) 65+/- 3.5 |
|
|
|
|
|
TA97 |
0 |
145 +/- 4.5 |
180 +/- 9.4 |
248 +/- 105.5 |
|
33 |
145 +/- 13.6 |
- |
- |
|
100 |
151 +/- 16.6 |
175 +/- 11.6 |
152 +/- 9.1 |
|
333 |
138 +/- 14.0 |
190 +/- 2.3 |
157 +/- 10.6 |
|
1000 |
149 +/- 1.0 |
195 +/- 4.3 |
158 +/- 6.3 |
|
3333 |
156 +/- 3.8 |
166 +/- 7.3 |
169 +/- 4.8 |
|
6666 |
- |
(c) 64 +/- 31.8 |
(c) 89+/- 7.7 |
|
|
|
|
|
TA98 |
0 |
22 +/- 2.3 |
2.9 +/- 4.9 |
26 +/- 2.0 |
|
33 |
20 +/- 2.4 |
- |
- |
|
100 |
18 +/- 1.5 |
32 +/- 2.2 |
24 +/- 2.6 |
|
333 |
23 +/- 3.0 |
26 +/- 3.2 |
25 +/- 1.0 |
|
1000 |
17 +/- 1.0 |
27 +/- 1.5 |
22 +/- 2.1 |
|
3333 |
19 +/- 0.3 |
16 +/- 2.3 |
18 +/- 1.2 |
|
6666 |
- |
(c) 0 +/- 0.0 |
(c) 5 +/- 2.1 |
a) S9 fractions prepared from the liver of Acrolor 1254-induced male Sprague-Dawley rats and male Syrian hamsters
b) Mean +/- standard error
c) Slightly toxic
Applicant's summary and conclusion
- Conclusions:
- Methyl methacrylate was tested in bacterial reverse mutation test in Salmonella typhimurium (Ames test) in 4 strains (TA97, TA98, TA100 and TA1535) at test concentrations of 33 - 6666 µg/plate. Slight cytotoxicity was observed at 6666 µg/plate.
Methyl methacrylate was not mutagenic in bacterial reverse mutation test in Salmonella typhimurium (TA97, TA98, TA100 and TA1535). - Executive summary:
Methyl methacrylate was tested in bacterial reverse mutation test in Salmonella typhimurium (Ames test) in 4 strains (TA97, TA98, TA100 and TA1535) at test concentrations of 33 - 6666 µg/plate. Slight cytotoxicity was observed at 6666 µg/plate. Methyl methacrylate was not mutagenic in bacterial reverse mutation test in Salmonella typhimurium strains TA97, TA98, TA100 and TA1535.
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