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EC number: 233-162-8
CAS number: 10049-04-4
Table 7.2.1/1: Table for Acute Toxicity
Number of dead /number of investigated
Time of death (range)
day 1 (1 animal) day 2 (1 animal)
Two male animals
day 2 (3 animals)day 3 (1 animal)
Two female, two male animals
93.86 (95% C.L. = 45.52 – 193.53)
In an acute toxicity study conducted according to the OECD guideline No. 401 and in compliance with GLP, Sprague-Dawley rats were administered Chlorine Dioxine (as a 0.2 % solution in water) by gavage at doses of 20, 40 and 80 mg/kg bw. Animals were then observed for 7 days for mortality, body weight changes. Gross autopsy was performed at the end of the experiment.
Death occurred in 2 males and 2 females treated at 80 mg/kg b.w. and in 2 males treated at 40 mg/kg b.w. within 24 -48 hours after the test substance administration. No rat died in the 20 mg/kg b.w. group.
Oral LD50combined = 93.86 mg/kg bw (95% C.L. of 45.52 -193.53 mg/kg bw).
Animals treated at the lowest dose (20 mg/kg b.w.) clinically showed hypoactivity, piloerection and hunched posture during the first three days of the study and then recovered.
Females treated at 40 mg/kg bw showed the same clinical signs up to day 5 of the study. Males treated at 40 mg/kg bw and all animals treated at the highest dose (80 mg/kg bw) showed clinical signs involving the CNS, the respiratory tract, and also piloerectoin, hunched posture and salivation. All reviving animals recovered within days 4 -7 of the study.
The autopsy of animals which died during the study showed test substance-related changes in the gastrointestinal tract: congestion and erosions with mucus or test substance presence in the stomach and thinning walls with catarrhal or catarrhal-hemorrhagic content in the intestine. Congestion of the lungs, of the kidneys and of the liver and decreased size of the spleen were also observed. Animals treated at 20 mg/kg bw didn't show any necroscopic changes at the autopsy performed at the end of the study.
The surviving animals belonging to the groups treated with the highest doses (40 and 80 mg/kg bw) showed changes in the stomach (thickening of the glandular mucosa) considered test substance related. There were no treatment related changes in body weight.
This acute oral study is classified as acceptable. It does satisfy with the guidelines requirements for an acute oral study (OECD 401) in the rats.
Under the test conditions, Chlorine dioxide at 0.2% is classified Category 3 (H301: Toxic if swallowed) according to the CLP regulation (1272/2008) and to the GHS, as the LD50 value is comprised between 50 and 300 mg/kg bw. Effects observed are related to the corrosive properties of ClO2.
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