Registration Dossier

Administrative data

Endpoint:
additional toxicological information
Type of information:
other: in vitro study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Entry adopted from the OECD SIAR on sulfur dioxide without modification.Study meets generally accepted scientific principles, well documented, specific investigation of mechanism of bronchoconstriction - in vitro study; study acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Sulfur dioxide and sodium metabisulfite induce bronchoconstriction in the isolated perfused and ventilated guinea pig lung via stimulation of capsaicin-sensitive sensory nerves
Author:
Bannenberg G, Atzori L, Xue J, Auberson S, Kimland M
Year:
1994
Bibliographic source:
Respiration 61: 130-137

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Study only reports on isolated mechanistic investigations:
Mechanism of bronchoconstriction - in vitro study.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): sulphur dioxide
- Physical state: gaseous

no further details stated

Results and discussion

Any other information on results incl. tables

Lungs of capsaicin-pretreated guinea pigs showed decreased sulphur dioxide-induced bronchoconstriction.  Release of CGRP neuropeptide: significantly increased from start to termination of exposure; no release from lungs of capsaicin-pretreated guinea pigs.

Bronchoconstriction, induced in isolated guinea pig lung by in vitro exposure to 250 ppm sulphur dioxide, was accompanied by the release of calcitonin gene-related peptide (CGRP) from capsaicin-sensitive sensory nerves into the pulmonary circulation.

Applicant's summary and conclusion

Conclusions:
Bronchoconstriction, induced in isolated guinea pig lung byin vitro exposure to 250 ppm sulfur dioxide, was accompanied by the release of calcitonin gene-related peptide (CGRP) from capsaicin-sensitive sensory nerves into the pulmonary circulation.