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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(2S)-5-amino-2-[(aminoacetyl)amino]-5-oxopentanoic acid
EC Number:
700-144-0
Cas Number:
13115-71-4
Molecular formula:
C7H13N3O4
IUPAC Name:
(2S)-5-amino-2-[(aminoacetyl)amino]-5-oxopentanoic acid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, non-adapted
Details on inoculum:
A fresh sample of activated sludge from the aeration basin of a municipal sewage treatment plant (Moers, Germany) was collected and aerated until usage. The inoculum was not fed during the aeration period. The duration of the aeration period was 1 day.

After determination of the suspended solids (dry weight) a sample containing approx.
1875 mg suspended solids was filtered by means of a water jet pump through a filter paper. The sludge was washed twice with mineral medium. Afterwards, it was suspended in 250 mL mineral medium and stirred until it was homogeneous. 4 mL of this suspension were used to inoculate 1 litre of mineral medium. This amount of inoculum corresponded to 30 mg/L suspended solids.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
0 d
Parameter:
% degradation (O2 consumption)
Value:
74
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
88
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
105
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
123
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
90 % degradation after 14 d
93 % degradation after 28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The test substance is considered to be readily biodegradable.