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EC number: 260-906-9
CAS number: 57693-14-8
EC50 (48h) between 10 and 100 mg/l.
Two studies according to OECD guideline
202 are available, both conducted under static conditions for an exposure
period of 48 h..
In one study, 5 concentrations were tested
in the range of 6.25 to 100 mg/l, prepared with dilution water in a
geometric series with a factor of 2. Twenty daphnids were exposed to
each concentration level and the control.
Concentrations of test item were
analytically verified by HPLC-DAD at the start of the
exposure (0 h) and at the end of the exposure (48 h) of all
concentration levels and the control.
Concentrations of test
item were successfully maintained for
the duration of the test, thus endpoints
were assessed based on nominal concentrations of test item.
criteria of the test guideline were fulfilled.
Effect levels after
EC10 = 16.4 mg/l
(95 % conf. limit 13.2 - 19)
EC50 = 30.2 mg/l
(95 % conf. limit 27.1 - 33.7)
EC100 = 100 mg/l
NOEC = 12.5 mg/l
LOEC = 25 mg/l (not
the second study, nominal
concentrations tested were 10 / 18 / 32 / 58 / 100 and 180 mg/I, and a
control without any additions. In all test concentrations, no alteration
of the test substance was observed after 48 hours.
the test, measured concentrations were in the range of 100 - 109 % of
the nominal values. Therefore, under test conditions, the test article
was sufficiently stable.
reported results are related to the average analytical concentrations of
the test article measured at the beginning and at the end of the test.
immobilization and abnormal behaviour of daphnids was observed in the
levels at 48 :
and EC0 = 10 mg/l
= 192 mg/l
= 79 mg/l.
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