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EC number: 249-079-5
CAS number: 28553-12-0
A tabulated summary of results is presented in the 'overall remarks and
attachments' window below.
No statistically significant differences were observed between the test
and vehicle control groups in the percentage of aberrant cells following
treatment with the test substance at 40, 80 or 160 µg/l, either with or
without metabolic activation, for the initial or repeat asssays.
Although there was a statistically significant trend of the series
without metabolic activation for the 20-hour harvest in the repeat
asssay, the response at the high dose (160 µg/ml) was not extreme and is
within the normal range of the vehicle control. It was not supported by
an observable dose response, and was not supported by other results at
other harvests. Examination of the replicates showed that one flask at
160 µg/ml, had 6 aberrant cells out of 100 while the other flask at the
same dose had only 2 aberrant cells out of 100. It appeared that the one
flask (6/100) was anomalous and not representative of the other data.
Based on this data analysis, there was no statistically significant
relationship between dose and the number of aberrant cells.
Chinese hamster ovary (CHO) cells were exposed to di-isononyl phthalate
(DINP) in acetone at concentrations of 5,10, 20, 40, 80 or 100 µg/ml
media, for 3 hours (with and without metabolic activation) in an initial
assay, or 3 hours (with metabolic activation) and 20 hours (without
metabolic activation) in a repeat assay, and harvested after 20 or 44
hours incubation, respectively. DINP did not induce chromosomal
aberrations in the cultured CHO cells at any of the evaluted dose levels
(40, 80 and 160 µg/ml) with and without metabolic activation.
The study was considered acceptable for classification, and satisfied
the requirements for mammalian cell chromosomal aberration studies.
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