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EC number: 271-974-4
CAS number: 68647-86-9
The adsorption coefficient KOC of charcoal (Probe 2: C-Fix = 80.5%), as read-across substance for charcoal, coconut shell, was determined using the HPLC method in accordance with the OECD guideline 121 and in compliance with GLP standards.
The study was
performed using HPLC with a cyanopropyl chemical bound resin on a silica
based column as stationary phase (pH = 5.5; 25 °C). As mobile phase
methanol/water (55/45% v/v) was used. Seven reference items with
different retention times (tR) were chosen for the
calibration on behalf of the retention time of the test item charcoal
(Probe 2: C-Fix =80.5%) on the column.
First, a solution of
the reference items was measured three times with HPLC, then a solution
of the test item (three measurements), and finally the solution of the
reference items was again measured three times.
For each reference
item, the capacity factor k’ was calculated from the retention time of
an unretained compound (sodium nitrate) and the retention time of the
respective reference item. A calibration function was set up using the
literature values for KOC of the reference items and the mean
capacity factor of the six determinations. Based on the data set log k’
versus log KOC, the equation for the regression
line was calculated.
The chromatogram of
the test item showed one peak (mean TR= 2.254 min). The log KOC
was calculated as 0.661±0.026 (mean ± standard deviation) with a
range of 0.633–0.686. As this log KOC-value lies
outside the calibration range obtained with the used reference
substances (1.32-5.63) and also outside the determinable log KOC-range
of the HPLC screening method (according to OECD guideline 121: 1.5-5.0),
the log KOC of the test item charcoal (Probe 2: C-Fix =
80.5%) should be stated as <1.5.
Due to the limited
solubility of the test item charcoal (Probe 2: C-Fix = 80.5%) in the
solvent, an extract instead of a solution was used for the measurements.
Therefore, the results are necessarily related to the used charcoal
extract with a nominal concentration of 208,910 mg/L in methanol/water
(75/25% v/v) under the testing conditions used (25±1°C; pH 5.5).
Further information on
adsorption/desorption according to REACh Regulation (EC) No. 1907/2006,
Annex IX, 9.3.3. column 1, is not required as charcoal is highly
insoluble in water and the overall potential of charcoal to become
adsorbed to soil/ sediment is extremely low (log KOC = 0.661;
see IUCLID section 5.4.1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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