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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The biodegradability of charcoal (Probe 2: C-Fix = 80.5%), as read-across substance for coconut shell charcoal, was tested according to OECD guideline 301B (CO2 evolution test) and under GLP compliance.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The test item was tested in a concentration of 25 mg/L. As positive control, aniline was tested in parallel. The biodegradation of the test substance was determined by measuring the emitted CO2concentrations on test days 2, 4, 7, 9, 11, 14, 18, 23, during the 28-day test period and on day 29. The biodegradation for the whole test period was determined as <2%. Hence, charcoal (Probe 2: C-Fix = 80.5%) is considered not readily biodegradable. For the positive control, a degradation of 64% was achieved within 9 d, ensuring the validity of the test.