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EC number: 246-690-9
CAS number: 25167-70-8
acute study test atmosphere characteristics of diisobutylene
Test atmosphere characteristics
Target concentration (ppm)
40 mL/kg CP
Nominal concentration (ppm)
Test article used (g)
Total volume chamber air (L)
Analytical concentration (mean)
Mean concentration of DIB-1(ppm)
Mean concentration of DIB-2 (ppm)
756 ppm (n=5)
21°C ± 0 (n=8)
24°C ± 0.7 (n=8)
22°C ± 0.5 (n=8)
25°C ± 0.7 (n=8)
46% ± 0.9 (n=8)
30% ± 1.2 (n=8)
34% ± 1.2 (n=8)
35% ± 0.7 (n=8)
= cyclophosphamide monohydrate
Based on the results of this study,
Diisobutylene at concentrations of 995, 2035 and 4015 ppm did not induce
a statistically significant increase in the incidence of micronucleated
polychromatic erythocytes in the bone marrow when male and female albino
rats were exposed to test article as a single, 4-hour, whole-body
2,4,4-trimethylpentene (purity >95%) was not
mutagenic to S. typhimurium or E. coli strains in the presence or
absence of metabolic activation at concentrations up to 5,000 ug/plate
(HLS, 1996 e). Similarly, diisobutylene (purity about 85%) was negative
a Bacterial Reverse Mutation Assay, using Salmonella typhimurium tester
strains TA98, TA100, TA1535 and TA1537 and Escherichia coli tester
strain WP2 uvrA in the presence and absence of Aroclor-induced rat liver
S9 (BioReliance, 2005).
In addition, 2,4,4-trimethylpentene
(purity>95%) was negative when tested in an in vitro chromosomal
aberration test using human peripheral blood lymphocytes in the presence
or absence of metabolic activation (HLS, 1997 d).
Diisobutylene (purity about 85%) did not
significantly increase the incidence of micronucleated polychromatic
erythrocytes in male or female rats exposed by inhalation to
concentrations of 0, 995, 2035 or 4015 ppm for 4 hours (WIL, 2006b).
Overall there is no evidence of mutagenic
activity with either 2,4,4-trimethylpentene (>95% purity) or
diisobutylene (about 85% purity) either in vitro or in vivo from the
core assay systems reported.
The evidence from in vitro and in vivo
studies indicate that 2,4,4 -trimethylpentene is not mutagenic and hence
classification under DSD or CLP is not warranted.
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