Trisodium 7-(4-(4-fluoro-6-(2-(2-vinylsulfonylethoxy)ethylamino)-1,3,5-triazine- 2-ylamino)-2-ureidophenylazo)naphthalene-1,3,6-trisulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 February 1987 to 06 February 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Holding : in dechlorinated tap water Test : in artifical water Temperature: lower temperature Limit Test : 1 concentration tested

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: Op. 1/86, Vers. 10/86

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the bath at the beginning and at the end of the experiment.

Vehicle:

no

Details on test solutions:

The test substance was dissolved in 200 mL water, which were slowly added to the fish tank.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Test organism Zebrafish (Brachidanio rerio)
- Weight: 0.19 g
- Length: 25.9 mm
- Age: 5 months
- Acclimation: 12 days in dechlorinated tap water
- Supplier: West Aquarium, Bad Lauterberg, BRD

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

100 mg/L (as CaCO3)

Test temperature:

21.9 - 22.9 °C

pH:

7.6 to 8.3

Nominal and measured concentrations:

Nominal: 1000 mg/L
Analytical: at start: 996.6; at end 1050.4 mg/L

Details on test conditions:

- Vessels: 5 liter glass aquaria
- Volume: 2.9 liter Test concentration: (3.0 liter minus 2x 0.05 liter for analytical control)
- Number of fish: 10 fish per concentration and water control
- Feeding: none
- Aeration: slowly aerated
- Lightening: 12 hours light / 12 hours darkness (yellow light TL 16/20 W)

In a pretest carried out at 10, 30, 100, 200 and 1000 ppm the LC50 was determined to be >1000 ppm. Therefore, the test concentration in the final (limit) test was chosen as 1000 ppm. A control was carried out with water (blank control).

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At the test concentrations used - 1000 ppm - (1000 mg/L), no mortalities were evident. An LC50 could therefore not be calculated.

Sublethal observations / clinical signs:

No abnormal responses and mortalities of the fish were observed.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 FAT 40224/C is >1000 mg/L to zebrafish.

Executive summary:

Acute toxicity to fish of the test substance was determined in a GLP-compliant study performed according to the OECD guideline 203. Ten zebrafish per dose were placed in a slowly aerated static system and exposed to 1000 mg/L of test substance for 96 hours. Water samples were taken from the fish tank at the beginning and at the end of the experiment to determine concentrations of the test substance present. No effects and mortalities were seen at the tested concentration, therefore the LC50 of FAT 40224/C was >1000 mg/L.

Description of key information

Acute toxicity to fish of the test substance was determined in a GLP-compliant study performed according to the OECD guideline 203. Ten zebrafish per dose were placed in a slowly aerated static system and exposed to 1000 mg/L of test substance for 96 hours. Water samples were taken from the fish tank at the beginning and at the end of the experiment to determine concentrations of the test substance present. No effects and mortalities were seen at the tested concentration, therefore the LC50 of FAT 40224/C was >1000 mg/L. The test substance is considered non-toxic to the test species.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L