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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Repeated dose toxicity: oral

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Administrative data

Endpoint:
chronic toxicity: oral
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
In the present investigation, a 44-day dosed feed study was performed to compare the availability of lead from a sample of lead-contaminated soil with a soluble lead salt, lead acetate, and a sparingly soluble lead salt, lead sulfide, as standards.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Lead sulphide
EC Number:
215-246-6
EC Name:
Lead sulphide
Cas Number:
1314-87-0
Molecular formula:
PbS

Test animals

Species:
rat
Strain:
Fischer 344
Sex:
male

Administration / exposure

Route of administration:
oral: feed
Vehicle:
not specified
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
44 days
No. of animals per sex per dose:
30
Control animals:
yes

Results and discussion

Effect levels

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Dose descriptor:
other: A-ALAD activity
Effect level:
ca. 127 ppm
Sex:
male
Basis for effect level:
other: A-ALAD activity was significantly decreased below control levels in all animals receiving lead in the diet and, in most cases, A-ALAD inhibition increased as the dose increased.
Dose descriptor:
other: Blood Lead Levels
Effect level:
ca. 127 ppm
Sex:
male
Basis for effect level:
other: Overall, blood lead concentrations were highest following administration of lead acetate, followed by lead sulfide and lead-contaminated soil.
Dose descriptor:
other: Bone lead concentrations
Effect level:
ca. 127 ppm
Sex:
male
Basis for effect level:
other: Administration of lead sulfide in the diet resulted in only 20 to 30% of the bone lead concentrations.

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

Exposure to lead was proportional to dose and, based on body weight growth patterns, did not result in overt toxicity.

It appears that not all exposures to lead pose the same risk. That is, lead bioavailability can vary greatly with the form and matrix in which lead is received.

The immature rat can be used as a very sensitive indicator of lead bioavailability from a variety of sources including contaminated soil.

Applicant's summary and conclusion

Conclusions:
In the conditions of the study, lead sulphide was found to have a low bioavailability in rat at repeated doses and thus did not result in overt toxicity.