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Diss Factsheets

Administrative data

Endpoint:
biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Test design not relevant for any toxicological endpoint. Basic data given.

Data source

Reference
Reference Type:
publication
Title:
Reduction of Induced Mutability with Xanthine- and Imidazole-derivatives through Inhibition of Metabolic Activation
Author:
Yamaguchi T. and Nakagawa K.
Year:
1983
Bibliographic source:
Agric. Biol. Chem., 47 (7), 1673-1677

Materials and methods

Principles of method if other than guideline:
Inhibitory test on mutagenicity: A modified Ames mutation assay was performed using Salmonella typhimurium TA 98 and TA 100 as test strains. Cells were treated with a known mutagen and a sample solution and the mutagenicity was tested by the standard method. The result was compared with a control in which distilled water was used instead of the sample solution.
GLP compliance:
no
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethyl-4-methylimidazole
EC Number:
213-234-5
EC Name:
2-ethyl-4-methylimidazole
Cas Number:
931-36-2
Molecular formula:
C6H10N2
IUPAC Name:
2-ethyl-4-methyl-1H-imidazole
Details on test material:
Name of test substance (as cited in study report): 2-Ethyl-4-methylimidazol

Test animals

Species:
other: Salmonella typhimurium
Strain:
other: TA 98, TA 100

Administration / exposure

Details on study design:
Cells of Salmonella typhimurium TA 98 or TA 100 were treated at 37°C for 20 min with 0.1 mL of mutagen, with or without 0.25 mL of S-9 mix and 0.1 mL of a sample solution and the mutagenicity was tested by the standard method. The result was compared with a control in which distilled water was used instead of the sample solution.

Results and discussion

Details on results:
In contrast to imidazole and 1-methylimidazole, 2-Ethyl-4-methylimidazole did not lead to a reduction in TRP-P-2, acetylaminofluorene and benzo(a)pyrene induced mutagenicity in Salmonella typhimurium TA100 and TA98.

Applicant's summary and conclusion