Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 603-006-7 | CAS number: 124728-62-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- Sep 01, 2009 - Oct 07, 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge, micro organisms from a domestic waste water treatment plant
- Origin: municipal sewage treatment plant
- Conditioning: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice.
- Concentration of suspended solids per litre: 30 mg - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- According to guideline
- Reference substance:
- aniline
- Test performance:
- The reference item (aniline) was sufficiently degraded to 70 % after 14 days and to 71 % after 28 days of incubation. The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 0
- Sampling time:
- 28 d
- Results with reference substance:
- 14 d: 70 %, 28 d: 71 %
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus, the test item is not readily biodegradable.
- Executive summary:
Purpose
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. The substance concentration during the course of this study was monitored by LC with UV detection (244 nm). As a reference item aniline was tested simultaneously under the same conditions as the test item and functioned as a procedure control. The GLP study was performed according to OECD TG 301C.
Result
Biodegradation of test item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 0 % as found. HPLC analysis confirmed that no degradation occurs.
Biodegradation of aniline
The reference item was sufficiently degraded to 70 % after 14 days and to 71 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Conclusion
The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus, the test item is not readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2010-08-12 to 2010-12-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- The inoculum was derived from the secondary effluent of Shanghai Longhua Sewage Plant. The inoculum was pre-conditioned by aerating the secondary eftluent, without other treatment or addition, for 5-7 days at the test temperature of 20 + 1 °C. Control microbe counted at the range of
10000-1000000 orgs/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Remarks:
- final concentration was clearly above water solubility
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 20 +/- 1 °C
- pH: 7.4
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration:
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: BOD bottles
- Number of culture flasks/concentration: 10
- Method used to create aerobic conditions: aeration of medium
- Test performed in closed vessels: yes
- Test performed in open system: no
SAMPLING
- Sampling frequency: on day 0, 7, 14, 21 and 28
- Sampling method: 2 bottles per group were taken for sampling every 7 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes - Reference substance:
- laurylsulfonate
- Remarks:
- sodium laurylsulfonate
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Under the test conditions no biodegradation of test item could be observed.
The percentage degradation did not exceed 60 % within the 10-day window and after 28 days of incubation.
The test item is considered not readily biodegradable.
In the toxicity control containing both, test item and reference item 77 % biodegradation was noted within 14 days and 80.5 % biodegradation was determined after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory on the activated sludge micro organisms because degradation was > 25 % within 14 days.
The oxygen demand in the abiotic control was zero; no correction of the test item degradation rates had to be done. - Results with reference substance:
- The reference item sodium laurylsulfonate was sufficiently degraded to 76.5 % after 14 days and to 79.5 % after 28 days of incubation. The percentage degradation of the reference item confirms the suitability of the used activated sludge inoculum.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under the test conditions no biodegradation of test item could be observed. The percentage degradation did not exceed 60 % within the 10-day window and after 28 days of incubation. The test item is considered not readily biodegradable.
- Executive summary:
In this OECD 301D Assay the results show no biodegradation of test item.
The percentage degradation did not exceed 60 % within the 10-day window and after 28 days of incubation.
The test item is considered not readily biodegradable.
The reference item sodium laurylsulfonate was sufficiently degraded to 76.5 % after 14 days and to 79.5 % after 28 days of incubation. The percentage degradation of the reference item confirms the suitability of the used activated sludge inoculum.
In the toxicity control containing both, test item and reference item 77 % biodegradation was noted within 14 days and 80.5 % biodegradation was determined after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory on the activated sludge micro organisms because degradation was > 25 % within 14 days.
The oxygen demand in the abiotic control was zero; no correction of the test item degradation rates had to be done.
Referenceopen allclose all
Description of key information
The test item is not considered to be readily biodegradable according to OECD 301D and OECD 301C.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Two experimental studies are available to address the endpoint for the substance. They are considered together.
OECD 301C
Purpose
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. The substance concentration during the course of this study was monitored by LC with UV detection (244 nm). As a reference item aniline was tested simultaneously under the same conditions as the test item and functioned as a procedure control. The GLP study was performed according to OECD 301C.
Result
Biodegradation of test item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 0 % as found. HPLC analysis confirmed that no degradation occurs.
Biodegradation of aniline
The reference item was sufficiently degraded to 70 % after 14 days and to 71 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Conclusion
The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus, the test item is not readily biodegradable.
OECD 301F
In this OECD 301D Assay the results show no biodegradation of test item. The percentage degradation did not exceed 60 % within the 10-day window and after 28 days of incubation. The test item is considered not readily biodegradable. The reference item sodium laurylsulfonate was sufficiently degraded to 76.5 % after 14 days and to 79.5 % after 28 days of incubation. The percentage degradation of the reference item confirms the suitability of the used activated sludge inoculum. In the toxicity control containing both, test item and reference item 77 % biodegradation was noted within 14 days and 80.5 % biodegradation was determined after 28 days of incubation. According to the test guidelines the test item can be assumed to be not inhibitory on the activated sludge microorganisms because degradation was > 25 % within 14 days. The oxygen demand in the abiotic control was zero; no correction of the test item degradation rates had to be done.
Based on the results of the studies the test item is considered not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.