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EC number: 452-330-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 October 2003 to 10 October 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A nominal 500 mg/L stock solution of the test material was prepared in deionised water. This stock was observed to be a clear, pale yellow solution after 60 minutes stirring and 30 minutes ultrasonic treatment and the pH was measured as 4.8. Test solutions were prepared by the addition of a known volume of this stock solution to a total volume of 500 mL aerated tap water, synthetic sewage and activated sludge.
- Controls: Two culture control flasks. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Method of cultivation: Activated sludge was obtained from Buckland Sewage Treatment Works, Newton Abbot, Devon, UK, 3 days prior to testing. The activated sludge was settled and the concentrated sludge was washed with tap water. The sludge was fed with 50 mL OECD synthetic sewage feed per litre of sludge per day and aerated at room temperature.
A synthetic sewage mixture was prepared containing the following per litre of deionised water:
15.2 g peptone
10.5 g meat extract
2.9 g of urea
0.7 g of NaCl
0.4 g of CaCl2.2H2O
0.2 g of MgSO4.7H2O
2.8 g of H2HPO4
- Total filterable solids: 7515 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.1
- Dissolved oxygen:
- At least 6.5 mg O2/L at the end of the 3 hour aeration period
- Nominal and measured concentrations:
- Nominal: 1.0, 3.2, 10, 32 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Flask
- Aeration: The flasks were aerated throughout the exposure period
- No. of vessels per control (replicates): Duplicate
- No. of vessels per concentration (replicates): Duplicate
- Final solids concentration: 1600 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water
OTHER TEST CONDITIONS
- Adjustment of pH: The pH was adjusted with hydrochloric acid
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rate of each culture was measured after 3 hours and compared with the mean respiration rate of the two control cultures. The rate of oxygen uptake was measured in a confined perspex cell into which a polargraphic oxygen electrode was inserted.The electrode was connected to a meter whose output was recorded on a potentiometric chart recorded. The dissolved oxygen concentration after the 3 hour period was at least 6.5 mg O2/L. The respiration rate was measured over the linear part of the curve for five to ten minutes. The rates for oxygen uptake were expressed at mg O2/L/h. The respiration rates of the flasks dosed with the test substance of the reference substance were expressed as percentages of the mean respiration rate of the control flasks, from which the percentage inhibition was derived:
% inhibition = (1-(Respiration rate of test flask/Mean respiration rate of control flasks)) x 100 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3,5-DCP)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? The results are valid
- Relevant effect levels: The reference substance caused substantial inhibition of the respiration rate of the activated sludge, From the results obtained, the 3 hour EC50 was estimated to be 4.8 mg/L. This is slightly less that the expected normal range of 5 to 30 mg/L indicating that the sludge was more sensitive than usual. As no inhibition was observed for the test substance , this is not considered to have affected the validity of the study. - Validity criteria fulfilled:
- not specified
- Remarks:
- The information on the dissolved oxygen concentration is not sufficient to assess the study against the criteria provided in the OECD guideline.
- Conclusions:
- Under the conditions of the test, <10 % inhibition was observed in all the tested concentrations. The 3 h EC50 of the test material was determined to be >100 mg/L. The 10 % effect concentration (EC10) is defined as the concentration resulting in a 10% reduction in the respiration rate of activated sludge within the period of the test. Percentage inhibition less than or equal to 10 % was within the expected experimental variability of the test and was considered not to be an effect of the test material. The NOEC under the conditions of this study is therefore equivalent to the EC10 in this study and was determined to be 100 mg/L. The study is considered to be reliable, relevant and adequate for risk assessment purposes.
- Executive summary:
The effect of the test material on the respiration rate of activated sludge was investigated in accordance with the standardised guidelines OECD 209 and EU Method C.11. Activated sludge was exposed to nominal concentrations of the test material of 1.0, 3.2, 10, 32 and 100 mg/L with controls and a reference substance (3,5-dichlorophenol) run concurrently for comparison. Less than 10 % inhibition was observed in all the tested concentrations and the 3 hour EC50 was determined to be >100 mg/L. The 10 % effect concentration (EC10) is defined as the concentration resulting in a 10% reduction in the respiration rate of activated sludge within the period of the test. Percentage inhibition less than or equal to 10 % was within the expected experimental variability of the test and was considered not to be an effect of the test material. The NOEC under the conditions of this study is therefore equivalent to the EC 10 in this test was determined to be 100 mg/L.
Reference
Table 2: Inhibition results
Test Substance |
Nominal Test Concentration (mg/L) |
pH |
Respiration rate (mg O2/L/h) |
Percentage Mean Control Respiration Ratea |
Percentage Inhibition |
|
Start |
End |
|||||
Control |
Control |
6.9 |
7.7 |
27.5 |
- |
- |
3,5-DCPb |
100 |
7.2 |
7.7 |
0 |
0 |
100 |
3,5-DCP |
32 |
7 |
7.7 |
2 |
7 |
93 |
3,5-DCP |
10 |
6.9 |
7.8 |
8 |
28 |
72 |
3,5-DCP |
3.2 |
6.9 |
7.9 |
18 |
63 |
38 |
3,5-DCP |
1 |
7 |
7.8 |
20.5 |
71 |
29 |
Test material |
100 |
6.9 |
7.6 |
27 |
94 |
<10 |
Test material |
32 |
6.9 |
7.6 |
29.5 |
102 |
<10 |
Test material |
10 |
6.9 |
7.7 |
29.5 |
102 |
<10 |
Test material |
3.2 |
6.9 |
7.7 |
31.5 |
109 |
<10 |
Test material |
1 |
7 |
7.7 |
30 |
104 |
<10 |
Control |
Control |
7 |
7.7 |
30 |
- |
- |
aMean control respiration rate = 28.8 mg O2/L/h b3,5-dichlorophenol |
Description of key information
3 h EC50 = > 100 mg/L, NOEC = 100 mg/L, OECD 209, Bowles & Maynard 2003
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
The effect of the test material on the respiration rate of activated sludge was investigated in accordance with the standardised guideline OECD 209. Activated sludge was exposed to nominal concentrations of the test material of 1.0, 3.2, 10, 32 and 100 mg/L with controls and a reference substance (3,5-dichlorophenol) run concurrently for comparison. Less than 10% inhibition was observed in all the tested concentrations and the 3 hour EC50 was determined to be >100 mg/L. The 10% effect concentration (EC10) is defined as the concentration resulting in a 10% reduction in the respiration rate of activated sludge within the period of the test. Percentage inhibition less than or equal to 10 % was within the expected experimental variability of the test and was considered not to be an effect of the test material. The NOEC under the conditions of this study is therefore equivalent to the EC 10 in this test was determined to be 100 mg/L. The available data is considered to be complete and the result determined, 3 h EC50 value ≥100 mg/L, was taken forward for risk assessment.
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