Trisodium 5-[[4-chloro-6-(ethylphenylamino)-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

Refer chapter 13 for the detailed analogue justification.

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: Inhouse SOP (method B.10) validated by Ciba which is comparable to standard guideline.

Deviations:

not specified

Principles of method if other than guideline:

Inhouse SOP (method B.10) validated by Ciba which is comparable to standard guideline.

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Remarks on exposure duration:

None

Reference substance (positive control):

not specified

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

The IC50 for FAT 40034/A was found to be greater than 300 mg/L.

Executive summary:

The toxicity of FAT 40034/A to activated sludge was determined in a 3-h respiration inhibition test according inhouse SOP (method B.10) validated by Ciba which is comparable to standard guideline. Based on the findings of the study, the IC50 of the test substance FAT 40034/A is greater than 300 mg/L.

Description of key information

The BST of the test substance Reactive Red 24:1(basis of source substance-FAT 40034/A) is greater than 300 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

300 mg/L

Additional information

The toxicity of FAT 40034/A (source substance) to activated sludge was determined in a 3-h respiration inhibition test according In house SOP (method B.10) validated by Ciba which is comparable to standard guideline.

So, based on source substance data, the BST of Reactive Red 24:1 is greater than 300 mg/L.