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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP-compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to guideline
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
GLP compliance:
yes (incl. QA statement)
Remarks:
Experimental Toxicology and Ecology, BASF Aktiengesellschaft, 67056 Ludwigshafen/Rhein, Germany

Test material

Constituent 1
Reference substance name:
297132-04-8
EC Number:
608-403-9
Cas Number:
297132-04-8
IUPAC Name:
297132-04-8
Details on test material:
- Name of test substance: 4-(4-Acryloyloxy-butoxycarbonyloxy)-benzoic acid
- Aggregate state/colour: solid / white
- Homogeneity: Yes
- Storage conditions: ambient (RT), Iight exclusion, Iimited storage: 24 months, dry storage

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test substance was added in the required amount to the test vessel with about 180 mL deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. Then synthetic medium was added the pH-value was adjusted to 7.5 ± 0.5, the inoculum was added and the test started.

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min

Test conditions

Test temperature:
20 ± 2°C
pH:
- Before correction: 5.8 - 6.8
- After correction: 7.3 - 7.6
Nominal and measured concentrations:
Nominal: 0 (control), 5.6, 32.0, 100.8, 506.8, 994.8 mg/L
Details on test conditions:
- Test vessels: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
- Oxygen concentration during aeration: > 2.5 mg/L
- Oxygen concentration immediately before measurement: > 6.5 mg/L
- Duration of the measurement of oxygen consumption: 8 - 10 min
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
ca. 700 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion