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EC number: 700-202-5
CAS number: 765-63-9
It was not possible to develop a method of analysis with sufficient
sensitivity to verify all of the exposure concentrations employed during
the study (limit of detection of the analytical method defined as 0.5
mg/L). Therefore, to enable the test results to be expressed in terms of
measured concentrations, the concentrations at 0.0954, 0.305 and 0.977
mg/L were calculated based on the measured levels of TFO at 3.13 and 10
At the start of the test, the measured levels in samples of the test
cultures at 3.13 and 10 mg/L were 53% and 68% of their nominal values
and after 72 hours, the measured levels were 49 and 64% of their nominal
values, respectively. These results give overall mean concentrations of
0.0558, 0.179, 0.572 (calculated), 1.60 and 6.59 mg/L (measured). After
72 hours, it was not possible to detect any TFO in a sample of medium
containing TFO at 0.0954 mg/L, which had been incubated without algal
cells; however, in a sample at 10 mg/L, the measured level increased
when compared to the initial concentration of TFO detected (83% of
nominal compared to 68% of nominal). These results indicate that the
concentrations of the test substance may have been influenced by the
presence of algal cells.
The pH of the control cultures increased by more than 1.5 pH units
during the 72-hour exposure period; the increase in pH observed during
the definitive test is not thought to have affected either the validity
or integrity of the study because the validity criteria for this type of
test were met by the control cultures.
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