[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: the only test concentration and the control
- Sample storage conditions before analysis: stored in a freezer (≤ - 20 °C), protected from light

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Strain/clone: clone 5
- Age at study initiation (mean and range, SD): From 2.0 to 17.0 hours old
- Source: in house laboratory culture
- Feeding during test no

ACCLIMATION
- Acclimation period: was not necessary, since the test was performed in the same medium as the culturing
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon
- Feeding frequency: all working days

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

19.3 to 20.1 °C in the freshly prepared media;
19.4 to 19.5°C in the aged test media

pH:

7.6 to 7.8 in the freshly prepared media;
7.7 to 7.8 in the aged test media

Dissolved oxygen:

8.9 to 9.1 mg/L in the freshly prepared media;
8.8 to 9.0 mg/L in the aged test media

Nominal and measured concentrations:

114.3 mg test item/L (corresponding to 100 mg a.i./L, when adjusted to water content)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Volume of solution:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Test water: Elendt "M4"

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Light intensity: 360 to 620 lux

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 114.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 114.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

All reported results refer to nominal values since the concentrations of the test item were within ± 20% of the nominal concentrations during the test.

Validity criteria fulfilled:

yes

Conclusions:

After 48 hours of exposure no immobilisation of the test animals was observed in the control and the only test item concentration of 100 mg/L.

Description of key information

EC50 (48 h): >100 mg/L (nominal, OECD202)