Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 609-461-8
CAS number: 376653-36-0
Density (OD) and the calculated Non Specific Colour % (NSCliving%)
of the Additional Control Tissues
Optical Density (OD)
blank value was 0.047
density means the mean value of the duplicate wells for each sample
(rounded to three decimal places).
Density (OD) and the calculated relative viability % of the samples
Viability (% RV)
Physiological saline (0.9% (w/v) NaCl)
Glacial acetic acid
(Glacial acetic acid)
Minimum optical density (OD)
Maximum optical density (OD)
Mean optical density (OD)
Standard deviation (SD)
Number of cases
optical density (OD) values measured are background corrected values
(measured at 570±30 mm)
An in vitro skin corrosivity
test of the test item BAL0001022 was performed in a reconstructed human
epidermis model. The model, EPISKINTM(SM) is designed to
predict and classify the corrosive potential of chemicals by measuring
its cytotoxic effect as reflected in the MTT
(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
(detailed in 3.6. section). The corrosivity of the test item was
evaluated according to the OECD No. 431 guideline.
Disks of EPISKINTM(SM)
were treated with BAL0001022 and incubated for 4 hours at room
temperature. Exposure of test material was terminated by rinsing with
Phosphate Buffered Saline solution. The viability of each disk was
assessed by incubating the tissues for 3 hours with MTT solution, to
evaluate cytotoxicity. The precipitated formazan crystals were then
extracted using acidified isopropanol and quantified
Physiological saline (0.9%
(w/v) NaCl solution) and glacial acetic acid treated epidermis were used
as negative and positive controls, respectively. Additional disks were
used to provide an estimate of colour contribution (NSCliving%) from the
test item. For each treated tissue viability was expressed as a %
relative to the negative control. If the mean relative viability after
4 hours of exposure is below 35% of the negative control, the test item
is considered to be corrosive to skin.
Following exposure to
BAL0001022, the mean cell viability was 104.9% compared to the negative
control, which is above the threshold of 35%, therefore the test item
was considered as being non-corrosive.
In conclusion, in this in
vitro EPISKIN™(SM) test model, the results indicate that BAL0001022 is
non-corrosive to skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again