(3-chloro-2-hydroxypropyl)trimethylammonium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Test duration too short, no information on the test substance given and documentation insufficient.

Principles of method if other than guideline:

Method: other: 10 animals/dose group; no further data

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Leuciscus idus melanotus

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 10 440 mg/L

Sublethal observations / clinical signs:

Result: LC50 = 9 ml/l

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Remarks:

Test procedure in accordance with national standard method with acceptable restrictions. REPORT NOT AVAILABLE.

Principles of method if other than guideline:

Method: other: DEV L15, DIN 38421, part 15 (1978)

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Leuciscus idus melanotus

Test type:

other: presumably static

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

11 600 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 11 600 mg/L

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Pimephales promelas

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2 000 mg/L

Reference 4

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

1982-09-16 to 1982-09-18

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Qualifier:

according to guideline

Guideline:

other: DIN 38 412 part 1

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

other: Idus melanotus

Water media type:

freshwater

Limit test:

no

Total exposure duration:

2 d

Duration:

2 d

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Validity criteria fulfilled:

not specified

Reference 5

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

only 48 h exposure

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

DIN 38412 Teil 15

Principles of method if other than guideline:

Method: other

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Leuciscus idus melanotus

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 900 mg/L

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 900 mg/L

Reference 6

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

Test procedure in accordance with national standard method. But 2 fish died at 1000 mg/l, whereas at 1500 mg/l no animal was killed. STUDY SEEMS TO BE SAME AS Scholtz, N. 1987: Bestimmung der akuten wirkungen von SERVON XRK 60 gegenüber Fischen (nach DIN 38412 Teil 15). Hüls aktiengesellschaft DUV-Ps/BT Prüfinstitut für Biologie. Study has been performed already in 1987!. NO STUDY REPORT FROM THE YEAR 1997 AVAILABLE.

Principles of method if other than guideline:

Method: other: according to DEV DIN 38412 part 15, 1982

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Leuciscus idus melanotus

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

1 500 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Reference 7

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No chemical analysis was performed.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

semi-static

Limit test:

no

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 3 200 mg/L

Sublethal observations / clinical signs:

After 96 h exposure at the highest concentration, the number of fish and their condition, visually assessed, were the same as those of the control fish. Therefore LC50 was higher than 3200 mg/l. The slope of the concentration-effect curve could not be calculated.

Reference 8

Endpoint:

short-term toxicity to fish

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Justification for type of information:

Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008).

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 3 200 mg/L

Conclusions:

After 96 h exposure at the highest concentration, the number of fish and their condition, visually assessed, were the same as those of the control fish. Therefore LC50 was higher than 3200 mg/l. The slope of the concentration-effect curve could not be calculated.

This information is used in a read-across approach in the assessment of the target substance.
Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008)

Reference 9

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Nominal and measured concentrations:

Nominal concentrations 1.000, 1.800, 3.200, 5.600 and 10.000 of QUAB 188 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

2 094 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4 128 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 6 544 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The LC50 values and their confidence interval were calculated by means of a parametric model developed by Kooijman (1981).

Sublethal observations / clinical signs:

NOEC 3200 mg (2094 mg/l), but in concentration 1800 mg/l (1178 mg/l) one fish died (the death is assumed to be accidental).

Validity criteria fulfilled:

yes

Conclusions:

A 96 h EC50 value of 4128 mg a.i./L has been determined for the effects of the test substance on mortality of Brachydanio rerio.

Description of key information

Eight short-term studies are available for fish. Seven studies were conducted with CHPTAC and one with its main degradation product 2, 3-Dihydroxy-N,N,N-trimethylpropan-1-aminium chloride. The determined effect concentrations are in a similar range and demonstrate that CHPTAC and its degradation product (diol) is not hazardous towards fish. The key value is determined as a 96-h LC50 of 4128 mg/L (nominal).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4 128 mg/L

Additional information

Overview of effect values:

Brachydanio rerio (new name: Danio rerio): 96-h LC50 = 4128 mg/L

Idus melanotus: 48-h LC0 = 1000 mg/L

Pimephales promelas: 96-h LC0 = 2000 mg/L

Leuciscus idus melanotus: 48-h LC0 = 11600 mg/L

48-h LC50 > 11600 mg/L

Leuciscus idus melanotus: 48-h LC0 = 1500 mg/L

48-h LC50 > 1500 mg/L

Leuciscus idus melanotus: 48-h LC50 = ca. 10440 mg/L

Leuciscus idus melanotus: 48-h LC0 > 900 mg/L

48-h LC50 > 900 mg/L

Effect concentration for the diol: Brachydanio rerio (new name: Danio rerio): 96-h LC50 > 3200 mg/L