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EC number: 928-779-0
CAS number: -
Table 1: Concentrations of the test item in
test medium (combined range-finding/limit test)
Table 2: Concentration of the test item in
test medium (extra test)
The limit of detection (S/N=3) was
determined to be 0.04 mg/l at an injection volume of 10µl (i.e. 0.4 ng
absolute). Taking into account the dilution factor of the sample, the
concentration of the test item in the 1 and 10 mg/l nominal samples (24
and 48 hour) has to be < 0.08 mg/l.
Stability during storage:
Extra samples were taken at t=0 h and
frozen for 2 days to examine the stability of the test item in test
medium during storage. For the 1, 10 and 100 mg/l nominal samples, the
relative to nominal concentrations were 78, 84 and 108 mg/l nominal
samples respectively when analysed freshly and 54, 78 and 119%
respectively when analysed after storage. From the results it was
concluded that the test item might be unstable in test medium during a
storage in a deep freeze for 2 days.
Table 3 and 4 show the analytical results of
the final test.
Calibration solutions of the
hydrolysis product of the test item were prepared at NOTOX B.V. from
standard solutions of the test item in ISO-medium. Because the chemical
structure of the hydrolysis product was not known, it was not possible
to express concentrations analysed as mg/l hydrolysis product.
Concentrations are therefore expressed as the equivalent test item
No test substance (test item) was
detected in the samples from the final test.
Table 3: Concentrations of the hydrolysis
product (peak 1) in test medium (final test)
Table 4: Concentrations of the hydrolysis
product (peak 2) in test medium (final test)
* Mean of duplicate analysis. The maximum
deviation between the responses was calculated for each sample and was
** Samples were frozen until analysis
*** Extrapolated from the regression line
**** Concentrations are not concentrations
of the hydrolysis product but concentrations of the equivalent test item
***** A small peak was detected with the
same retention time as peak 1
n.d. not detected
n.a. not applicable
Results Final Test:
Analysis indicated that during the
24-hour incubation period all test substance was converted in its
Recoveries based on peak 1 in the
samples taken from the degradation product of the test item prepared a
nominally 1.0 and 10 mg/l remained fairly stable between 128 and 169%
during the 48 hour exposure period. The recovery in the sample taken
from 0.22 mg/l increased from 74 to 302% of nominal.
Analysis based on peak 2 showed that
measured concentrations were below the limit of detection at nominal
0.22 and 1.0 mg/l. The concentration measured at nominal 10 mg/l
remained stable at approximately 1 mg test item equivalents per litre.
The results of analysis should be
interpreted with care, as the analytical method used was not a specific
method and ws not validated. Hence, it can be concluded that testing
involved the degradation products of test item only.
The responses recorded in this test allowed
for reliable determination of an EC50. The responses recorded were in
agreement with what was expected based on the results of the range
Table 5: Acute immobilisation of daphnia
after 24 and 48 hours in the final EC50 -test.
*Degradation products of test item
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