1,6-hexanediyl bismethacrylate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997-10-30 to 1997-11-05

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN EN ISO 10712 (1), 1995

Deviations:

no

Principles of method if other than guideline:

The test is performed with bacteria of the genus Pseudomonas which grow in the presence of an easily degradable substrate. Upon addition of a
toxic concentration of a test material the growth rate decreases. The growth rate is determined by turbidimetry (436 nm; 1 cm cuvette). The
extinction coefficients were transformed into Formazin­Nephelometric-Units (FNU). Five concentrations (5 dilutions of a stock solution of 1 g/1) of the test material were tested. The concentrations at which the bacterial growth rate is decreased by 10 % (EC 10) and 50 % (EC 50), respectively, as
compared to the average value of untreated controls, are determined, if possible.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,6-hexanediyl bismethacrylate

EC Number:

229-551-7

EC Name:

1,6-hexanediyl bismethacrylate

Cas Number:

6606-59-3

Molecular formula:

C14H22O4

IUPAC Name:

6-[(2-methylprop-2-enoyl)oxy]hexyl 2-methylprop-2-enoate

Details on test material:

- Name of test material (as cited in study report): 1,6-Hexanediol dimethacrylate
- Substance type: organic
- Physical state at room temperature: liquid

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Study design

Test type:

static

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

23 ± 1°C

Nominal and measured concentrations:

50, 100, 200, 400, and 800 mg 1,6-HDDMA/L (1.45, 2.9, 5.8, 11.6 and 23.2 mg 1,6-HDDMA/L concentration based on DOC-content)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer-flask
- Aeration: no
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
- Incubation period: 5h ± 0.5h
- Stock solution: 1 g/L

Reference substance (positive control):

yes

Remarks:

 3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

16 h

Dose descriptor:

EC0

Effect conc.:

800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Up to the highest nominal test concentration of 800 mg/L, 1,6-HDDMA had no effects (neither inhibition nor stimulation) on the growth of Pseudomonas putida.

Results with reference substance (positive control):

- Results with reference substance valid?: Yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The EC50 of 3,5-Dichlorphenol was in the accepted range of 10 (0.23 % growth inhibition) to 30 (97.55 % growth inhibition) mg/L.

Conclusions:

In a valid growth inhibition test according (Pseudomonas putida) to DIN EN ISO 10712 (1), 1995 after a contact time of 16 h, the EC0 was determined to be greather than 800 mg/L. Up to the highest nominal test concentration of 800 mg/L, 1,6-HDDMA had no effects (neither inhibition nor stimulation) on the growth of Pseudomonas putida.

Executive summary:

In a valid growth inhibition test according (Pseudomonas putida) to DIN EN ISO 10712 (1), 1995 after a contact time of 16 h, the EC0 was determined to be greather than 800 mg/L. Up to the highest nominal test concentration of 800 mg/L, 1,6-HDDMA had no effects (neither inhibition nor stimulation) on the growth of Pseudomonas putida.

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