Registration Dossier

Physical & Chemical properties

Water solubility

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2017-07-26 to 2017-08-10
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 830.7840 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
flask method
Key result
Water solubility:
987 g/L
Conc. based on:
test mat.
Incubation duration:
>= 24 - <= 72 h
Temp.:
20 °C
pH:
ca. 0
Details on results:
Preliminary Test
The water solubility of the test item was estimated to be 1114 g/L in a preliminary test.
Calibration
The calibration solutions were prepared in 0.5 – 50 μg/mL range. The calibration samples were analysed by HPLC-UV method with three replicate injections from each. The concentration of the test item was calculated with the help of the calibration equation.

Results of the determination of water solubility of 5-sulfosalicylic acid dihydrate

Sampling time

Measured concentrationofTest Item
(g/L )

Measured pH

Test vessels

Mean withthe 95% confidence intervals

After 24h

962

970 ± 16.9

< 0

976

< 0

971

< 0

After 48h

996

998 ± 10.0

< 0

1002

< 0

995

< 0

After 72h

993

994 ± 23.6

< 0

985

< 0

1004

< 0

Solubility ofthe test item (g/L):

987 ± 37.6

-

RSD%:

2

-

pH of ultra-pure water:

-

6.44

Conclusions:
The water solubility of the test item was determined to be 987 ± 37.6 g/L (95% confidence interval) at 20 °C ± 0.5 °C using the shake-flask method. The result is also applicable for the water free form of the test item.
Executive summary:

The water solubility was determined according to OECD 105, Regulation (EC) No. 440/2008 method A.6 and OPPTS 830.7840 using the shake flask method with HPLC analysis. About 80 mg test item were weighed into 100 mL stoppered Erlenmeyer flasks (nine parallels) according to the estimated solubility based on the Preliminary Test. After the addition of 25 mL ultra-pure water, the flasks were agitated at 30 °C. After one day, three of the vessels were removed and re-equilibrated at 20 °C for 24 hours. The content of the vessel was then centrifuged at 20 °C and the concentration in the clear aqueous phase was determined by HPLC. The other flasks were treated similarly after initial equilibration at 30 °C for two and three days, respectively. The samples were diluted before the analysis (two parallel dilutions). The pH of each sample was recorded. The water solubility of the test substance was determined to be 987 ± 37.6 g/L (95 % confidence interval) at 20 °C ± 1 °C (pH <0). The result is also applicable for the water free form of the test item.

Description of key information

The water solubility of the test item was determined to be 987 ± 37.6 g/L (95% confidence interval) at 20 °C ± 0.5 °C using the shake-flask method. The result is also applicable for the water free form of the test item.

Key value for chemical safety assessment

Water solubility:
987 g/L
at the temperature of:
20 °C

Additional information

The water solubility was determined according to OECD 105, Regulation (EC) No. 440/2008 method A.6 and OPPTS 830.7840 using the shake flask method with HPLC analysis (reference 4.8-1). About 80 mg test item were weighed into 100 mL stoppered Erlenmeyer flasks (nine parallels) according to the estimated solubility based on the Preliminary Test. After the addition of 25 mL ultra-pure water, the flasks were agitated at 30 °C. After one day, three of the vessels were removed and re-equilibrated at 20 °C for 24 hours. The content of the vessel was then centrifuged at 20 °C and the concentration in the clear aqueous phase was determined by HPLC. The other flasks were treated similarly after initial equilibration at 30 °C for two and three days, respectively. The samples were diluted before the analysis (two parallel dilutions). The pH of each sample was recorded. The water solubility of the test substance was determined to be 987 ± 37.6 g/L (95 % confidence interval) at 20 °C ± 1 °C (pH <0). The result is also applicable for the water free form of the test item.