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EC number: 207-353-1
CAS number: 464-45-9
1. Summary of results.
Mean OD /disc (#)
Mean OD / product
# mean of 3 values (triplicate of the same extract)
OD: optical density
*The optical density was measured after a 1:2
dilution of the formazan extracts in isopropanol.
Acceptability criteria: SD≤18%.
An in vitro skin irritation test of the test item was performed in
a reconstructed human SkinEthic RHE® model, according
to OECD TG 439 (GLP study). Three epidermis units were treated with 16
mg test item for 42 minutes at room temperature. Exposure of the test
item was terminated by rinsing with25 x 1 mL of DPBS.The
epidermis units were then incubated at 37°C for 41 hours 47 minutes in
an incubator with 5% CO2. The viability of each disk was assessed by
incubating the tissues with MTT, extracting the precipitated formazan
crystals using isopropanol during 2 hours under gentle agitation in the
dark, and measuring the concentration of formazan by determining the OD
at 570 nm, just after dilution of the extracts 1:2 in isopropanol. Under
test conditions, the mean percent viability of the treated
tissues was 5.6%, versus 1.2% in the positive control (5% Sodium Dodecyl
Sulfate). Therefore, the test item must be considered as skin irritant
(cat 2) or skin corrosive (Cat 1).
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