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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Reason / purpose for cross-reference:
read-across source
Remarks on exposure duration:
Medium change at 48 and 72 hr
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
There was no mortality during the exposure period in either the control or the 100 mg/L treatment.
Reported statistics and error estimates:
Not applicable, the results were based on observation of the data.
Sublethal observations / clinical signs:

Nominal concentration (mg/L)

Timepoint (h)

Measured concentration (mg/L)

Replicate 1

Replicate 2

Mean

100

0

99.91

100.92

100.42

100

4

96.65

98.59

97.62

100

24 (old)

99.47

97.38

98.43

100

24 (new)

93.58

95.07

94.33

100

48 (old)

97.26

85.89

91.58

100

48 (new)

94.98

95.92

95.45

100

72 (old)

81.63

80.89

81.26

100

72 (new)

106.33

99.34

102.84

100

96 (old)

104.22

96.82

100.52

Overall mean

-

-

-

95.07

The limit of quantification was 3.55 mg/L. All of the control measurements were less than the LOQ.

Validity criteria fulfilled:
yes
Conclusions:
The 96-hour LC50 determined was >100 mg succinic acid/L (nominal concentration). The NOEC (no-observed effect concentration) was 100 mg succinic acid/L.
Executive summary:

In a 96-h acute toxicity study, juvenile zebrafish (Danio rerio) were exposed to a single succinic acid concentration of 100 mg/L under semi-static conditions (48-h and 72-h media change).  The 96-h LC50 was > 100 mg /L.  The NOEC value, based on mortality, was 100 mg/L.  No sublethal effects were observed.  The test design included an untreated control comprising of dilution water only. The concentration of succinic acid was verified analytically, and the mean measured concentration was 95.07 mg/L. Since this was within 80 % of the initial concentration, nominal exposure concentrations were used throughout the duration of the test.


This toxicity study is classified as acceptable and satisfies the guideline requirements for OECD 203 acute fish toxicity studies. Based on the results of this study, succinic acid does not need to be classified in regards to environmental hazards according to Regulation (EC) No 1272/2008 (Part 4) and Directive 67/548/EEC


Results synopsis


Test organism size/age: 40 weeks, 3.21 cm (SD: 0.21)


Test type: Semi-Static (media change 48 and 72hr)


LC50: > 100 mg/L


NOEC: 100mg/L


Endpoint(s) effected: Mortality


This information is used in a read-across approach in the assessment of the target substance. For details and justification of read-across please refer to the read-across report attached to IUCLID section 13.

Description of key information

The potential toxicity of succinic anhydride to aquatic invertebrates is assessed via read-across from its hydrolytical transformation product succinic acid.


In the key study on the acute toxicity of the hydrolysis product (succinic acid) of succinic anhydride to fish according to OECD 203 (Ipser, 2010), juvenile zebrafish (Danio rerio) were exposed to a single succinic acid concentration of 100 mg/L under semi-static conditions (48-h and 72-h media change). The 96-h LC50 was > 100 mg /L and the NOEC value, based on mortality, was 100 mg/L, both corresponding to 85 mg/L succinic anhydride. No sublethal effects were observed.


Based on the results of this study, succinic anhydride does not need to be classified in regard to environmental hazards according to Regulation (EC) No 1272/2008 (Part 4) and Directive 67/548/EEC.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information