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EC number: 276-333-2
CAS number: 72089-08-8
The measured concentrations of the test item at the start of
exposure (0 h) were in the range of 63 – 72% of the nominal test item
concentrations and at the end of the exposure (72 h) in the range of 49
– 105% of the measured initial values. The geometric mean measured
concentrations are: 0.128, 0.457, 1.43, 5.16, and 17.2 mg/L
Table 1. Measured concentrations of the test item during the
Nominal test item concentration
Measured conc. [mg/L]
Geometric mean measured test item concentration [mg/L]
Meas.conc. = measured concentration of the test item
(dilution factor taken into account)
% = percentage of the initially measured
concentration of the test item
LOQ = Limit of quantification (0.01 mg test
1) = Reanalyzed 2 d later, mean value of
2) = Reanalyzed due to technical reasons 7
d later of the extracted and derivatized samples: the samples were
considered stable, therefore occurred no conflict with the expiry date
of the test item. (A potential degradation of the derivatized product is
alleviated since the derivatization of calibration levels and the test
level concentration occurred on the same day. If an aging of the samples
occurs, calibration levels and test concentrations will show the same
Table 2. Evaluation after 72 h. Statistically significant
differences of growth rates and yield compared to control values are
marked (+), not significant differences are marked (-).
Geometric mean measured test item concentation [mg/L]
Growth rate [d^-1]
Inhibition of growth rate [%]
< LOQ = below limit of quantification
n.a. = data not applicable
The toxicity to aquatic algae was assessed in static test according to
OECD guideline 201. P. subcapitata was exposed for 72 h to four dilution
levels with a spacing factor 3.16 of a saturated solution prepared with
a nominal loading rate of 25.5 mg test item/L. The test item
concentrations wree analytically verified at the beginning and end of
The measured initial concentrations ranged from 0.183 to 17.7 mg/L. The
measured concentrations at the end of the test were 49 - 105% of the
initially measured concentrations. The test resulted in an ErC10 (72 h)
of 1.01 mg/L and an ErC50 (72 h) of 6.49 mg/L.
ErC10 (72 h) = 1.01 mg/L (geometric mean
measured, P. subcapitata, OECD 201)
ErC50 (72 h) = 6.49 mg/L (geometric mean
measured, P. subcapitata, OECD 201)
There is one study available, in which
the toxicity of the substance to aquatic algae was investigated
according to OECD guideline 201 and GLP.
In a static test, P. subcapitata at an
initial cell density of 6839 cells/mL was exposed to 1.0, 3.16, 10.0,
31.6, and 100% of a saturated solution with a nominal loading of 25.5
mg/L test item for 72 h. The test item concentrations were analytically
verified by GC-MS at the beginning (0 h) and at the end (72 h) of the
The measured initial concentrations (at 0 h)
were in the range of 63 to 72% of the nominal test item concentrations
and at the end of the exposure (72 h) in the range of 49 to 105% of the
measured initial values. The geometric mean measured concentrations are
0.128, 0.457, 1.43, 5.16, and 17.2 mg/L.
After 72 h, the test item was found to
inhibit the growth of the freshwater green algae and the following
effect concentrations were obtained: ErC10 (72 h) = 1.01 mg/L and ErC50
(72 h) = 6.49 mg/L (geometric mean measured concentration).
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