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EC number: 276-333-2
CAS number: 72089-08-8
The biodegradation in % was calculated based on a ThOD of 3.02 mg O2/mg
The pass level for ready biodegradability (> 60%) was reached on Day 3.
The validity criterion that the degradation should be > 60% after 14 d
In the toxicity control the biodegradation was 47% after 14 d. After 28
d the biodegradation reached 57%. The validity criterion that the test
item should not inhibit the biodegradation of the reference item was
The ready biodegradability of the test item was determined with
non-adapted activated sludge in a manometric respirometry test for a
period of 28 d according to OECD guideline 301 F. The test item
concentration was 19.9 mg/L (60.0 mg O2/L). Both test item replicates
did not reach the 10% level (begin biodegradation phase) within the 28 d
period. The mean biodegradation on Day 28 was 4%.
Not readily biodegradable: 4% in 28 d (activated sludge, domestic,
non-adapted, OECD 301 F)
There is one GLP study available investigating the ready
biodegradability of the test substance according to the OECD guideline
In a Manometric Respirometry Test, a test medium with 19.9 mg/L
test item (nominal, 60.0 mg O2/L) was inoculated with 1.11E+07 colony
forming units/L non-adapted activated sludge from a local, municipal
sewage treatment plant and incubated for 28 d. A functional, inoculum,
as well as a toxicity control were run in parallel. Oxygen depletion by
the respiration of bacteria was monitored using the OxiTop system. The
percentage biodegradation was expressed in terms of biological oxygen
demand (BOD) and theoretical oxygen demand (ThOD).
The functional control reached the pass level (> 60%) on Day 3 and
maximum biodegradation (100%) on Day 25. In the toxicity control 47%
degradation occurred by Day 14 and 57% by Day 28. Therefore, the
degradation of the reference item was not inhibited by the test item.
Both replicates of the test item did not reach the 10% level (end
of adaptation, begin of biodegradation phase) within the 28 d period of
the test. The mean biodegradation of the test substance on Day 28 was
4%. Hence, the test item is classified as not readily biodegradable
within the 28 d period of the study.
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