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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Adequacy of study:
other information

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Siehe Bemerkungen.
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
431-460-4
EC Name:
-
Cas Number:
10061-02-6
Molecular formula:
C3H4Cl2
IUPAC Name:
Trans 1,3-dichloropropene
Test material form:
liquid
Details on test material:
Batch no: TR 88001
Also know as ST088/098

Method

Species / strain
Species / strain / cell type:
bacteria, other: sat TA 98, 100, 1535, 1537, 1538, esc WP2 uvrA; pKM101
Metabolic activation system:
Aroclor 1254 induced rat liver S9-homogenate.
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 39.1 ... 2500 µg/plate
Concentration range in the main test (without metabolic activation): 39.1 ... 2500 µg/plate
Vehicle / solvent:
Solvent: Acetone
Details on test system and experimental conditions:
Concentration of the test substance resulting in precipitation: 2500 µg/plate

Results and discussion

Test resultsopen allclose all
Species / strain:
other: as specified above
Metabolic activation:
with
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(>= 1250 µg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(>= 625 µg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(>= 1250 µg/plate)
Remarks on result:
other: other: preliminary test
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

toxicity: with S-9 mix from 1250 μg/plate; without S-9 mix from 625 μg/plate upwards. 


precipitations: with and without S-9 mix at the highest tested dose of 2500 μg/plate. 


 


characterisation of the positive finding: positive with and without S-9 mix in the dose range 312.5 to 1250 μg/plate; reproducible and dose-dependent effects. 

Applicant's summary and conclusion

Conclusions:
Interpretation of results:
positive with metabolic activation
positive without metabolic activation

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