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Administrative data

Endpoint:
acute toxicity: inhalation
Adequacy of study:
other information

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: 84/449/EWG, B.2; OECD 403 (1989)
GLP compliance:
yes
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
431-460-4
EC Name:
-
Cas Number:
10061-02-6
Molecular formula:
C3H4Cl2
IUPAC Name:
Trans 1,3-dichloropropene
Test material form:
liquid
Details on test material:
Batch no: TR 88001
Also know as ST088/098

Test animals

Species:
other: rat, Sprague-Dawley Crl:CD(SD)BR
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: aerosol
Vehicle:
other: None.
Details on inhalation exposure:
Head only
Duration of exposure:
4 h
No. of animals per sex per dose:
5

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
5.098 mg/L air
95% CL:
4.54 - 6.092
Exp. duration:
4 h
Mortality:
Male: 3.859 mg/L; Number of animals: 5; Number of deaths: 1
Male: 4.449 mg/L; Number of animals: 5; Number of deaths: 1
Male: 4.98 mg/L; Number of animals: 5; Number of deaths: 3
Male: 6.36 mg/L; Number of animals: 5; Number of deaths: 3
Female: 3.859 mg/L; Number of animals: 5; Number of deaths: 1
Female: 4.449 mg/L; Number of animals: 5; Number of deaths: 0
Female: 4.98 mg/L; Number of animals: 5; Number of deaths: 3
Female: 6.36 mg/L; Number of animals: 5; Number of deaths: 5
Clinical signs:
other: Signs of toxicity related to dose levels: 3.859 mg/l: On days 2 and 3 all animals exhibited piloerection, lethargy and abnormal fur. One female exhibited cold/hunched posture and loss of condition on day 5 and/or 6. Two animals died on days 4 and 6
Gross pathology:
Effects on organs:
Two from dose group 3.859 mg/l, one from dose group

4.449 mg/l, six from dose group 4.980 mg/l and eight from
dose group 6.360 mg/l rats died or were killed in extremis
during the course of the study. The most consistent necropsy
findings were dark/red and inflated lungs suggestive of
local effects on the respiratory tract. Yellow/dark contents
were also found in the stomach of five animals, and the
kidneys of two decedents were pale; possibly suggesting
effects outwith the respiratory tract.

Most treated animals surviving to study termination were
either unremarkable at necropsy or had minor lesions similar
to those of the controls. One dose group 4.449 mg/l female
had a pale zone in the corticomedullary region in the
kidney. Taken together with the pale kidneys in two of the
decedents, this was also suggestive of effects on the
kidneys.

Any other information on results incl. tables

Migrated information
On
the basis of the test results given below and in conformity with the criteria given in Annex VI of the Directive, the substance should be not classified X


Criteria used for interpretation of results: EU

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met