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Administrative data

Endpoint:
acute toxicity: oral
Adequacy of study:
other information

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method B.1 (Acute Toxicity (Oral))
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
431-460-4
EC Name:
-
Cas Number:
10061-02-6
Molecular formula:
C3H4Cl2
IUPAC Name:
Trans 1,3-dichloropropene
Test material form:
liquid
Details on test material:
Batch no: TR 88001
Also know as ST088/098

Test animals

Species:
other: rat, Fischer 344

Administration / exposure

Vehicle:
other: No vehicle.
No. of animals per sex per dose:
5

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
94 mg/kg bw
95% CL:
82 - 108
Remarks on result:
other: Slope of the mortality curve: 6.8
Mortality:
Male: 75 mg/kg bw; Number of animals: 5; Number of deaths: 0
Male: 90 mg/kg bw; Number of animals: 5; Number of deaths: 0
Male: 120 mg/kg bw; Number of animals: 5; Number of deaths: 4
Male: 160 mg/kg bw; Number of animals: 5; Number of deaths: 5
Female: 75 mg/kg bw; Number of animals: 5; Number of deaths: 2
Female: 90 mg/kg bw; Number of animals: 5; Number of deaths: 4
Female: 120 mg/kg bw; Number of animals: 5; Number of deaths: 4
Female: 160 mg/kg bw; Number of animals: 5; Number of deaths: 5
Clinical signs:
Signs of toxicity related to dose levels:
The principle signs of reaction to treatment were diarrhoea
or voiding of soft faeces, cyanosis, increased lachrymation,
a hunched posture, lethargy, piloerection and ante-mortem
prostration. Other clinical signs included abasia, increase
salivation and ataxia among rats does at 90 mg/kg and above
and an isolated case of ante-mortem hypothermia.
Piloerection, a hunched posture and an unkempt appearance
were commonly apparent on day 2 and day 3 among rats treated
at the low or intermediate dose-levels. The recovery of rats
surviving the toxic effects of trans-1,3-dichloroproperne
was generally advanced by day 4 although the unkempt
appearance of many rats persisted for up to nine days after
treatment.
Gross pathology:
Effects on organs:
Necropsy of decedents revealed similar abnormalities at each
dose level. Common pathological changes included
discoloration of the renal medulla, congestions of the
lungs, darkening of the liver, the presence of dark or light
patches on the liver, exaggeration of the hepatic lobular
pattern, petechiae on thymus and liquid contents and/or
inflammation of the stomach. Other isolated findings
included heamorrhage of the stomach, heamorrhage or
inflammation of the caecuum, pallor of the spleen and pale,
raised areas on the kidneys.

Any other information on results incl. tables

Migrated information


On the basis of the test results given below and in conformity with the criteria given in Annex VI of the Directive, the substance should be classified as: toxic X


Criteria used for interpretation of results: EU

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria