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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione
EC Number:
700-064-6
Cas Number:
2105830-60-0
Molecular formula:
Not applicable as this is a UVCB substance
IUPAC Name:
Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione
Constituent 2
Reference substance name:
[TN]Multiester P97-463[/TN][SPEC][/SPEC][AM]100 %[/AM]
IUPAC Name:
[TN]Multiester P97-463[/TN][SPEC][/SPEC][AM]100 %[/AM]
Details on test material:
Multiester P97-463 was a very viscous amber liquid. Multiester P97-463 was tested in duplicate
at approximately 34 mg per 2 litres, corresponding to 12 mg TOC/l. The organic carbon content
was based on the composition and structure. Preparation was as much as possible performed
under yellow light and/or dimmed light conditions.
In a pre-test Multiester P97-463 was not sufficiently soluble to allow preparation of an aqueous
solution at a concentration of 1 g/l. Therefore, Multiester P97-463 was weighed on glass slides
and quantitatively added to the test bottles (2 litres) containing medium with microbial organisms
and mineral components (test substance bottle A: 33.7 mg; test substance bottle B: 33.9 mg
and toxicity control bottle: 32.2 mg). The test solutions were continuously stirred during the test,
to ensure optimal contact between the test substance and the test organisms.

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
29 d
Initial test substance concentration
Initial conc.:
17 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
2 - 14
Sampling time:
29 d
Details on results:
Determination of ‘ready’ biodegradability: carbon dioxide (CO2) evolution test (modified Sturm test) with Multiester P97-463.
The study procedure was based on EEC directive 92/69, C.4-C, December 1992, OECD guideline No. 301 B July 17, 1992 and
ISO Standard 9439 (1999). Multiester P97-463 was a very viscous amber liquid. Multiester P97-463 was tested in duplicate at
approximately 34 mg per 2 litres, corresponding to 12 mg TOC/l. The organic carbon content was based on the composition
and structure. Preparation was as much as possible performed under yellow light and/or dimmed light conditions. The
Theoretical CO2 production (ThCO2) of Multiester P97-463 was estimated to be 2.60 mg CO2/mg.
In a pre-test Multiester P97-463 was not sufficiently soluble to allow preparation of an aqueous solution at a concentration
of 1 g/l. Therefore, Multiester P97-463 was weighed on glass slides and quantitatively added to the test bottles (2 litres)
containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during
the test, to ensure optimal contact between the test substance and the test organisms. The relative degradation values calculated
from the measurements performed during the test period revealed 14 and 2% degradation of Multiester P97-463, for the duplicate
bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
In the toxicity control, Multiester P97-463 was found not to inhibit microbial activity. Since all criteria for acceptability of the test
were met, this study was considered to be valid. In conclusion, Multiester P97-463 is designated as not readily biodegradable.

Any other information on results incl. tables

Reference substance

Identification number RS186

Name Sodium acetate

Description White powder (determined at NOTOX)

Molecular formula CH3COONa (taken from label)

Molecular weight 82.03 (taken from label)

Batch number K34333668

Article number 1.06268.0250

Purity 99.0%

Expiry Date 28 February 2010

Certified Yes

Storage conditions At room temperature in the dark

Supplier Merck, Darmstadt, Germany

Reference substance concentration and preparation of test solutions

A solution of sodium acetate was prepared by dissolving 402.0 mg in Milli-RO water and making

this up to a total volume of 100 ml. Volumes of 20 ml from this stock solution were added to 2

litres of the test medium of the positive control bottle and the toxicity control bottle, resulting in a

final concentration of 40 mg sodium acetate per litre (12 mg TOC/l).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Multiester P97-463 was not readily biodegradable under the conditions of the modified Sturm
test presently performed.
Executive summary:

SUMMARY

Determination of ‘ready’ biodegradability: carbon dioxide (CO2) evolution test (modified Sturm

test) with Multiester P97-463.

The study procedure was based on EEC directive 92/69, C.4-C, December 1992, OECD

guideline No. 301 B July 17, 1992 and ISO Standard 9439 (1999).

Multiester P97-463 was a very viscous amber liquid. Multiester P97-463 was tested in duplicate

at approximately 34 mg per 2 litres, corresponding to 12 mg TOC/l. The organic carbon content

was based on the composition and structure. Preparation was as much as possible performed

under yellow light and/or dimmed light conditions. The Theoretical CO2 production (ThCO2) of

Multiester P97-463 was estimated to be 2.60 mg CO2/mg.

In a pre-test Multiester P97-463 was not sufficiently soluble to allow preparation of an aqueous

solution at a concentration of 1 g/l. Therefore, Multiester P97-463 was weighed on glass slides

and quantitatively added to the test bottles (2 litres) containing medium with microbial organisms

and mineral components. The test solutions were continuously stirred during the test, to ensure

optimal contact between the test substance and the test organisms.

The relative degradation values calculated from the measurements performed during the test

period revealed 14 and 2% degradation of Multiester P97-463, for the duplicate bottles tested.

Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day

window) was not met.

In the toxicity control, Multiester P97-463 was found not to inhibit microbial activity.

Since all criteria for acceptability of the test were met, this study was considered to be valid.

In conclusion, Multiester P97-463 is designated as not readily biodegradable.