Acrylonitrile

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

publication

Title:

Acute toxicological evaluation of acrylonitrile (1969)

Author:

Vernon PA, Dulak LH & Deskin R

Year:

1990

Bibliographic source:

Journal of American College of Toxicology (1990):1, 114-115

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

: scores for erythem and oedema combhined; intact and abraded skin sites used; 24-hour exposure

GLP compliance:

no

Remarks:

Tests prior to GLP

Test material

Test material information

Constituent 1

Reference substance name:

Acrylonitrile

EC Number:

203-466-5

EC Name:

Acrylonitrile

Cas Number:

107-13-1

Molecular formula:

C3H3N

IUPAC Name:

prop-2-enenitrile

Test animals

Species:

rabbit

Strain:

New Zealand White

Test system

Type of coverage:

occlusive

Preparation of test site:

other: sites were shorn, intact and abraded sites were used

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

0.5 ml

Duration of treatment / exposure:

24 hours

Observation period:

72 hours

Number of animals:

Six

Results and discussion

In vivo

Results

Irritation parameter:

other: combined mean score for oedema and erythema

Basis:

mean

Time point:

other: 24-72h

Score:

3.6

Reversibility:

not fully reversible within: 72h

Any other information on results incl. tables

The mean score both erythema and oedema was 3.6, with slightly higher scores reported for abraded skin.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The results of this study indicate that acrylonitrile is a skin irritant, but is not corrosive.

Executive summary:

In this guideline-comparable study, 0.5 mL acrylonitrile was applied for 24 hours under occlusive conditions to the shorn (intact and abraded) dorsal skin of six New Zealand White rabbits. Dermal reactions were assessed at 24 and 72 hours following application and mean scores (24 and 72 hour) scores (on a scale of 0 to 4) for both erythema and oedema are reported. The mean score for both erythema and oedema was 3.6, with slightly higher scores reported for abraded skin. This study that acrylonitrile is a skin irritant and should be classified as such.