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EC number: 425-220-8
CAS number: 5945-33-5
The tests on Daphnia magna were conducted over a 48-hour period, using a
static limit test methodology. As with the fish test above the test
solution was prepared using dimethylformamide. Four replicate tests were
conducted using ten Daphnia in each test vessel, together with a solvent
control, using ten test animals. During the tests the temperature was
controlled at 21± 1°C, while the pH was always between 7.9 and 8.1,
dissolved oxygen between 7.7 and 8.2 mg/L and water hardness around 100
mg/L as CaCO3. Analysis of duplicate samples of the BDP in water after
the 48-hour test period found concentrations of 1.03 and 1.06 mg/L,
which was around 105% of the nominal concentration. No immobilisation of
any of the 40 test Daphnia was observed during the course of the test,
nor was any other aberrant behaviour observed. These results indicate
that the BDP is not toxic to this species up to the limits of its water
A limit test on the inhibition of algal growth was also conducted on
Selanastrum subspicatus over a 72-hour incubation period at 21 ± 1℃
with nominal concentration of the test material of 1.0 mg/L. The
test material was introduced to the solutions in the manner indicated in
the studies above. Six replicate tests were conducted in 250 mL
Erlenmeyer flasks, together with three control flasks containing no
chemical. Three solvent controls (i.e. water and the appropriate amount
of dimethylformamide) were also run. Each flask contained 100 mL of the
test medium, and the flasks were continuously agitated to maintain the
algal cells in suspension. The pH of the test solutions increased from
around 8.0 at the start of the period, to around 10 after 72 hours. The
growth of algal biomass was determined over the test period by
measurement of cell density using absorbance at 665 nm. At 0 hours the
mean cell density in the control was 1.85 x 104 cell/mL,
increasing to 2.17 x 106 cells/mL after 72 hours. The
corresponding values for the solvent control were 2.17 x 104
and 1.3 x 106 cell/mL, respectively. While the report did not
detail the actual cell densities for the test media, the absorbance
closely paralleled that of the controls (and solvent control), and
consequently it was concluded that the BDP is not inhibitory to growth
of algae up to the limits of its water solubility.
A test on the inhibition of bacterial respiration was also conducted.
The test substance was suspended in artificial sewage at nominal
loadings of 20, 500 and 1,000 mg/L using a 30 minute sonication to
assist dispersion. The test flasks were inoculated with sewage sludge
bacteria and aerated for 30 minutes. Following aeration the contents of
the flasks were poured into darkened 300 mL BOD bottles fitted with
oxygen sensing electrodes. The rate of oxygen consumption was measured
for the dispersions, and compared with the vessel. None of the tests
indicated any significant inhibition of bacterial respiration compared
with the controls, and it was concluded that the BDP is not toxic to
sewage bacteria up to the limits of its water solubility.
The acute test on rainbow trout was a Static Limit Test (no replacement
of test water) performed over 96 hours using a solution of the test
substance made up at a nominal concentration of 1 mg/L in charcoal
filtered dechlorinated tap water. The test was performed in duplicate
using ten fish in each test vessel. A solvent control was also run in
parallel, also using ten fish. During the tests the temperature was
controlled at 14 ± 1oC, while the pH was always between
7.7 and 8.0, dissolved oxygen between 9.4 and 10.1 mg/L and water
hardness around 100 mg/L as CaCO3. All test specimens
survived over the 96 hour test period. Further, no physical or
behavioural anomalies were observed during the test period, and
accordingly it was concluded that the BDP is non toxic to rainbow trout
up to the limits of its water solubility.
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