1-(5,6,7,8-tetrahydro-3,5,5,6,8,8-hexamethyl-2-naphthyl)ethan-1-one

Additional ecotoxological information

Administrative data

Endpoint:

additional ecotoxicological information

Adequacy of study:

key study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Data included and protocol for detection of AHTN in plants developed. Probable cross contamination from soil to leaves

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Developed protocol for the determination of AHTN in plant materials.

Principles of method if other than guideline:

The analysis of AHTN is described in a seperated protocol that was included as an Annex in the study (Anhang B5-1)

GLP compliance:

not specified

Type of study / information:

The absorption of AHTN in lettuce and carrots in various AHTN spiked sewage sludge amended soils.

Test material

Test material information

Constituent 1

Reference substance name:

1-(5,6,7,8-tetrahydro-3,5,5,6,8,8-hexamethyl-2-naphthyl)ethan-1-one

EC Number:

216-133-4

EC Name:

1-(5,6,7,8-tetrahydro-3,5,5,6,8,8-hexamethyl-2-naphthyl)ethan-1-one

Cas Number:

1506-02-1

Molecular formula:

C18H26O

IUPAC Name:

1-(5,6,7,8-tetrahydro-3,5,5,6,8,8-hexamethyl-2-naphthyl)ethan-1-one

Details on test material:

AHTN - Tonalid; no further specifications.

Results and discussion

Any other information on results incl. tables

The method has been validated. The accuracy in the measurement according to DIN 32 645 (1994), taking into account sample processing, is 1 µg/kg. The correctness of the procedure is based on the AHTN recovery rate, which is 98%.

Lettuce

The soil analysis for the soil/sewage mixture at the different locations has been measured over a period of 12 weeks (Table 3).

	Greenhouse				Outdoor
Week	Borstel	Friesland	Latrop	Control	Borstel
0	37.2	29.3	31.8	n.d.	37.2
6	25.1	26.3	28.5	n.d.	28.4
8	26.8	31.0	30.7	n.d.	28.1
10	28.2	30.3	30.8	n.d.	30.4
12	30.6	36.4	35.4	n.d.	33.1
Average	29.6	30.6	31.4	n.d.	31.4

Table 3 Average AHTN content (mg/kg dw) in the test soils for the lettuce (n.d. non-detectable, detection limit 0.025 mg/kg dw).

Table 4 shows the AHTN content in the lettuce as function of time.

	Greenhouse				Outdoor
Week	Borstel	Friesland	Latrop	Control	Borstel	Control
6	584	384	215	47	854	28
8	183	218	239	30	667	2
10	226	207	191	101	957	67
12	107	198	190	21	476	13
Average	275	252	209	50	738	27

Table 4 Average AHTN concentration (µg/kg dw) in lettuce over the exposure period of 12 weeks.

The AHTN content in the soil remained constant and no degradation in the soil was observed. The average AHTN concentration was 245 µg/kg dw. No accumulation of AHTN was observed. Lettuce in the greenhouse showed a similar concentration around 0.2mg/kg dw after 8 weeks. The outdoor values are higher and this could possibly be due to rain splash from the AHTN spiked soil.

Carrots

Analysis of the soil for the test of AHTN in root and leaves of carrots in the greenhoue can be found in the table below.

Week	Friesland	Latrop	Control
0	29.3	31.8	n.d.
14	29.7	34.7	n.d.
16	35.6	35.9	n.d.
18	37.8	35.6	n.d.
Average	33.1	34.5	n.d.

Table 5 Average AHTN concentration (µg/kg dw) in lettuce over the exposure period of 12 weeks(n.d. non-detectable, detection limit 0.025 mg/kg dw).

The absorption of AHTN in carrot roots and leaves is shown in table 6

	Root (mg/kg dw)				Leaves (µg/kg dw)
Week	Friesland	Latrop	Control	Friesland	Latrop	Control
14	4.5	18.0	n.d.	189	1290	12
16	1.8	8.6	n.d.	75	1210	18
18	1.8	11.8	n.d.	82	212	13
Average	2.7	12.8	n.d.	115	902	15

Table 6 AHTN absorption in carrot roots (in mg/kg dw) and leaves (µg/kg dw)

In contrast to the lettuce, there is a dependency on the soil type.