Registration Dossier
Registration Dossier
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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
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EC number: 207-274-2 | CAS number: 457-68-1
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Completed by a CGLP laboratory to an approved methodology.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- The SkinEthic HCE model consists of transformed human keratinocytes of the cell line HCE (LSU EYE Center) that form a corneal epithileal tissue (mucosa), devoid of stratum corneum, resembling, histologically, the mucosa of the human eye. The test material is applied directly to the culture surface, at the air interface, so that undiluted and/or end use dilution can be tested directly. The test is based on the hypothesis that irritant chemicals are able to penetrate the stratum corneum of the SkinEthic HCE model and are sufficiently cytotoxic to cause cel death in the underlying cell layers.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,1'-methylenebis[4-fluorobenzene]
- EC Number:
- 207-274-2
- EC Name:
- 1,1'-methylenebis[4-fluorobenzene]
- Cas Number:
- 457-68-1
- Molecular formula:
- C13H10F2
- IUPAC Name:
- 1-fluoro-4-[(4-fluorophenyl)methyl]benzene
Constituent 1
Results and discussion
In vivo
Results
- Irritation parameter:
- other: Tissue viability
- Basis:
- mean
- Remarks:
- Relative mean tissue viability
- Time point:
- other: 10mins
- Score:
- ca. 106
- Max. score:
- 100
- Reversibility:
- not specified
- Remarks on result:
- other: in vitro study showing no signs of irritation
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
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