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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28.06.2000-30.06.2000
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DS/ISO 14669 (1999): Water Quality - Determination of acute lethal toxicity to marine copepods
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
(1R,3S)-2,2-Dimethyl-3-(2-oxopropyl)cyclopropanecarboxylic acid
Cas Number:
70223-33-5
Molecular formula:
C9H14O3
IUPAC Name:
(1R,3S)-2,2-Dimethyl-3-(2-oxopropyl)cyclopropanecarboxylic acid
Test material form:
solid: crystalline

Test solutions

Details on test solutions:
0, 1, 2, 5, 10, 20, 50 & 100 mg/l

Test organisms

Test organisms (species):
other: Arcartia tonsa
Details on test organisms:
TEST ORGANISM
- Common name: Marine copepod
- Strain: Arcartia tonsa
- Source: Originally from the North Sea, now cultured at DHI

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20º
pH:
8.0
Salinity:
31 ‰
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml glass beaker (Pyrex)
- Fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 6


TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Natual Sea water obtained south east of Anholt in a depth of 25 m.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light: 8 hours dark

TEST CONCENTRATIONS
- Test concentrations: 0, 1, 2, 5, 10, 20, 50, 100 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol, testet every 3 month

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC10
Effect conc.:
19.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: -16.7-40.8
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
62.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 41.3-114
Details on results:
In the test at 20 mg/l one beaker was found to have a mortality at 100%, compared to the others with a max of 1 out of 5 death. There is a suspicion that this beaker was poluted. Because of the unsertainty about the cause of the observed mortality at 20 mg/l it was decided to use the whole dataset. This result in a somehow conservative estimat of the toxicity of the substance.
Results with reference substance (positive control):
- Results with reference substance valid? Yes
The sensitivity of the animals was tested with 3,5 -dichlorphenol and calculation showed an LC50(48h) 1.27 mg/l. This is in between the normal findings (LC50: 0.5 -1.5 mg/l). The sensitivity of the animals is assessed to be normal.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Mortality in controls < 10 %, oxygen > 60%, mortality with reference test within acceptable range: 0.5-1.5 mg/l
Conclusions:
The substance is found to have a LC50 (48h): 62.3 mg/l for Arcartia tonsa. (This is considered to be a very conservative result)