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Diss Factsheets
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EC number: 204-673-3 | CAS number: 124-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- At the beginning and end of exposure
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 +- 2°C
- Nominal and measured concentrations:
- nominal: 120, 69.8, 40.6, 23.6, 13.8, 8.0 mg/L (common ratio: 1.7) and control group
- Details on test conditions:
- - Initial cell concentration: 1x10^4 cells/mL
- Exposure period: 72 h
- Culture method: shake cultur (100 rpm)
- Number of vessels: 3 vessels per concentration
- Test volume: 100 mL/ vessel
- Test water temperature: 23 +- 2°C
- Lighting: 4000 - 5000 lux (continuous lighting) - Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 40.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 64.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The results relate to nominal concentartion based on test substance concentrations
- Conclusions:
- After 72 h a EC50 of 64.5 mg/L and a NOEC of 40.6 mg/L were determined based on growth rate for adipic acid towards algae.
- Executive summary:
A study was conducted in accordance with OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test' to assess the toxicity of the test substance towards algae after 72 h.
Exponentially growing algal cells (Pseudokirchnerella subcapitata, previously Selenastrum capricornutum) were exposed for a period of 72 hours to a range of concentrations, nominally 120, 69.8, 40.6, 23.6, 13.8, 8.0 mg/L of adic acid and a control. During the test a temperature range of 21 - 25 °C was maintained in the test vessels.
After 72 h a EC50 of 64.5 mg/L and a NOEC of 40.6 mg/L were determined based on growth rate for adipic acid towards algae.
Reference
Description of key information
A study was conducted in accordance with OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test' to assess the toxicity of the test substance towards algae after 72 h.
Exponentially growing algal cells (Pseudokirchnerella subcapitata, previously Selenastrum capricornutum) were exposed for a period of 72 hours to a range of concentrations, nominally 120, 69.8, 40.6, 23.6, 13.8, 8.0 mg/L of adic acid and a control. During the test a temperature range of 21 - 25 °C was maintained in the test vessels.
After 72 h a EC50 of 64.5 mg/L and a NOEC of 40.6 mg/L were determined based on growth rate for adipic acid towards algae.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 64.5 mg/L
- EC10 or NOEC for freshwater algae:
- 40.6 mg/L
Additional information
Preparing the SIDS-dossier within the scope of the ICCA/HPV-program the study conducted by the National Institute of Technology and Evaluation, Japan was not available. The BASF study was taken into account.
Toxicity to algae was assessed using effect concentration relating to biomass obtained in a study conducted to the EU-method C.2. According to the Guidance on information requirements and chemical safety assessment R.7b (ECHA, 2008) results based on the biomass should not be used for assessment since biomass results depend on the growth rate of the species as well as the test duration and other elements of the test design. Thus, the presented result was scored as not reliable. Reconsiderations results in an 72-h EC50 of 39.8 mg/l and an 72-h EC10 of 14 mg/l.
This study on aquatic algae was characterized by a concentration-dependant decrease of pH. At the end of the 72-hour exposure period, the pH in the control treatment was 10.1, whereas it decreased from 8.2 to 5.4 in the range of concentrations (31.3 - 62.5 mg/L) covering the EC50 value (39.8 mg/L). Furthermore, it should be noticed that the pH value in the control treatment is high compared to the guideline's requirements. Based on this observation, a pH effect cannot be ruled out when considering the toxicity reported in this study.
Describing the toxicity towards aquatic algae, the study conducted by the National Institute of Technology and Evaluation, Japan is preferred, despite deficiencies in study documentation concerning pH.
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