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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May - Jul 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Principles of method if other than guideline:
not relevant
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
6.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 5.9 - 7.2
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
18.6
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 13.0 - 24.1

Table 1: Inhibition of the growth of Scenedesmus subspicatus after 72 hours exposure to ZK 156454

Concentration (mg/L)  Inhibition in percent
of ZK 156454   biomass (inteqral) growth rate
0.0  0 0
3.1 28.7 9.4
6.2  47.1  18.1
12.5  69.8 31.6
25.0  87.0 59.8
49.9  94.4  86.6
Validity criteria fulfilled:
yes
Conclusions:
ZK 156454 had an inhibitory, concentration-related effect on the growth of Scenedesmus subspicatus. The EbC50 (biomass) was 6.5 (5.9-7.2) mg/L, the ErC50 (growth rate) was 18.6 (13.0-24.1) mg/L, including the 95% confidence limits.
Executive summary:

The purpose of this study was to determine the effects of the test compound Triamin.Dihydrochlorid (ZK 156454) on the

growth of the green algae Scenedesmus subspicatus. The study was conducted in agreement with the OECD guideline no. 201.

The test substance ZK 156454 was incubated in an aqueous solution including nutrients with an algae population of Scenedesmus subspicatus for a test duration of approximately 72 hours.

The nutrient solution was made up of mainly nitrate, phosphates and some trace elements. The test concentrations were 0, 3.1, 6.2, 12.5, 25.0 and 49.9 mg/I (nominal). The algae were exposed to each concentration in triplicate. Six vessels were prepared for the control. The algae were incubated in an incubator shaker under continuous light.

As a parameter for the growth of the algae population, the cell concentrations of the test and control solutions were counted with an electronic particle counter ("Coulter Counter"). The increase of biomass and the growth rate were calculated on the basis of the cell counts. The calculated biomass and growth rate of each concentration were compared to those of the controls, and the inhibition was calculated. ZK 156454 had an inhibitory, concentration-related effect on the growth of Scenedesmus subspicatus.

Description of key information

ZK 156454 had an inhibitory, concentration-related effect on the growth of Scenedesmus subspicatus. The EbC50 (biomass) was 6.5 (5.9-7.2) mg/L, the ErC50 (growth rate) was 18.6 (13.0-24.1) mg/L, including the 95% confidence limits.

Key value for chemical safety assessment

EC50 for freshwater algae:
6.5 mg/L

Additional information