α,α'-propylenedinitrilodi-o-cresol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1967

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Principles of method if other than guideline:

The test demonstrates the toxicity of an atmosphere enriched with the volatile parts of the test substance at 20 °C. Young adult laboratory rats, 3-6 per sex, were exposed sequentially to the vapors generated by blowing 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 30 minutes or 1 or 3 hours. The exposure was subsequently repeated in the same manner. No analytical determination of the atmosphere concentrations was performed. The nominal concentration was calculated as quotient of the amount of test substance weight loss during exposure, and the amount of air used during exposure. Group-wise documentation of clinical signs was performed over a 7 day study period. Body weight of groups was determined before the start of the study and at the end of the observation period. Gross necropsy of the animals was performed after death of the animals or at the end of the study.

GLP compliance:

no

Test type:

other: Inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

α,α'-propylenedinitrilodi-o-cresol

EC Number:

202-374-2

EC Name:

α,α'-propylenedinitrilodi-o-cresol

Cas Number:

94-91-7

Molecular formula:

C17H18N2O2

IUPAC Name:

α,α'-propylenedinitrilodi-o-cresol

Details on test material:

- Name of test material (as cited in study report): Keromet MD
- Physical state: liquid
- Analytical purity: ca. 80 %
- Impurities (identity and concentrations): toluene, ca. 20 %

Test animals

Species:

rat

Strain:

other: US-rats

Sex:

male/female

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: toluene

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

>= 30 - <= 180 min

Concentrations:

30 min exposure: ca. 52 mg/l (probably mostly toluene)
60 min exposure: ca. 41 mg/l (probably mostly toluene)
180 min exposure: ca.16 mg/l (probably mostly toluene)

No. of animals per sex per dose:

3 males and 3 females for the 30 and 60 mins of exposure
6 males and 6 females for the 180 mins of exposure

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology

Statistics:

no data

Results and discussion

Mortality:

0/12 animals died after 30 mins of exposure, 1/6 animals died after 60 mins of exposure, 2/6 animals died after 180 mins of exposure

Clinical signs:

other: attempts to escape, irritation of mucous membranes, narkosis due to toluene solvent

Body weight:

no data

Gross pathology:

some animals with chronic bronchitis, otherwise organs without findings