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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22 April - 31 May, 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
yes
Remarks:
Protocol amendments are described in section "Overall remarks, attachments".
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
α-trimethylsilanyl-ω-trimethylsiloxypoly[oxy(methyl-3-(2-(2-methoxypropoxy)propoxy)propylsilanediyl]-co-oxy(dimethylsilane))
EC Number:
406-420-4
EC Name:
α-trimethylsilanyl-ω-trimethylsiloxypoly[oxy(methyl-3-(2-(2-methoxypropoxy)propoxy)propylsilanediyl]-co-oxy(dimethylsilane))
Cas Number:
69430-40-6
Molecular formula:
Unspecified example: C18.3H46.8O5.8Si4.1
IUPAC Name:
α-trimethylsilanyl-ω-trimethylsiloxypoly[oxy(methyl-3-(2-(2-methoxypropoxy)propoxy)propylsilanediyl]-co-oxy(dimethylsilane))
Details on test material:
Identification: DC 5067
Description: Clear, light brown liquid
Lot Number: AB 090122
Purity: >99%
Storage conditions: In the original container at room temperature in the dark
Stability under storage conditions: Stab1e
Expiry date: December 1, 1991

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks:
(90% at nominally 1000 mg/l, 20% at 560 mg/l)
Remarks on result:
other: 95% CL: 407 to 1810 mg/l

Any other information on results incl. tables

Sublethal observations / clinical signs:

After 96 hours of exposure a total of 90% mortality was recorded at nominally 1000 mg/l, while 20% mortality was recorded at 560 mg/l. No biologically significant lethal effects in rainbow trout were observed at or below nominally 320 mg/l. Effects other than mortality, were mainly observed in fish exposed to 560 mg/l and higher. The most important effects recorded were discolouring and/or hypoactivity.

The analytical results could not be quantified owing to the complex composition of DC 5067. The chromatographic patterns of the samples taken from the fresh media were comparable with the chromatographic patterns of the samples taken from the 24-hour old media, except for some minor differences.

The LC50 values calculated from these data decreased from >l000 mg/l at 24 hours to 656 mg/l at 96 hours with a 95% confidence interval of 407 to 1810 mgll. The slope of the concentration/response curve at 96 hours was 5.16.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test DC 5067 induced no biologically significant lethal effects in rainbow trout at or below nominally 320 mg/l with a 96h-LC50 of 656 mg/l (95 % confidence interval between 407 and 1810 mg/l).
Executive summary:

96-hour Acute Toxicity Study in the Rainbow Trout with DC5067.

Ten fish per concentration were exposed for a maximum of 96 hours in a semi-static system to a concentration range for nominally 100 to 1000 mg/l.

After 96 hours of exposure a total of 904 mortality was recorded at nominally 1000 mg/l, while 20% mortality was recorded at 560 mg/l. No biologically significant lethal effects in rainbow trout were observed at or below nominally 320 mg/l. Effects other than mortality, were mainly observed in fish exposed to 560 mg/l and higher. The most important effects recorded were discoloring and/or hypoactivity.

The LC50 values calculated from these data decreased from >l000 mg/l at 24 hours to 656 mg/l at 96 hours with a 95% confidence interval of 407 to 1810 mg/l. The slope of the concentration/response curve at 96 hours was 5.16.

This study has been examined and determined not reportable under TSCA 8(-e).