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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 16-July 1, 2020
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2020
Report date:
2020

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(Z)-non-6-enal
EC Number:
218-900-9
EC Name:
(Z)-non-6-enal
Cas Number:
2277-19-2
Molecular formula:
C9H16O
IUPAC Name:
6-Nonenal, (6Z)-
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: all concentrations
- Sample storage conditions before analysis: Samples were placed in glass vials with formic acid, and then diluted with Elendt M4 medium to 5 mL. 50 uL of internal standard was then added, and the sample extracted with hexane.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added to brown sterile bottles and Elendt M4 medium was added with a sterilized stir bar. The test solution was then stirred for 72 hrs. It was then allowed to settle for 1 hr. Since the test substance had a higher density than water, it settled to the bottom of the flask. The settled test substance in the bottom 200 mL was drawn off, and the test solution taken from the higher water layer saturated with test substance.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): At higher concentration some precipitate may have formed.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age at study initiation (mean and range, SD): 4-24 hrs old
- Source: in-house
- Age of parental stock (mean and range, SD): 3 weeks
- Feeding during test : None

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None

Test conditions

Test temperature:
18-22 degrees C
pH:
7.72-8.56
Dissolved oxygen:
8.33-8.97 mg/L
Nominal and measured concentrations:
Nominal: 1.05, 2.32, 5.10, 11.2, 24.7 mg/L
Measured:
Details on test conditions:
TEST SYSTEM
- Test vessel: Conical flasks
- Type (delete if not applicable): covered with air-permeable silicone sponge caps
- Material, size, headspace, fill volume: 100 mL
- Volume of solution: 100 mL
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium

OTHER TEST CONDITIONS
- Adjustment of pH: 7.72-8.45
- Light intensity: 833-841 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility at 0, 24, and 48 hrs.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9.06 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3.07 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: At 48 hrs the daphnids in the 8.16 mg/L concentration were less mobile than controls.
- Mortality of control: None
- Immobilisation of control: None
Reported statistics and error estimates:
95% confidence limit of EC50: 7.14-11.3 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-hr EC50 for daphnids was 9.06 mg/L.
Executive summary:

An OECD Guideline 202 aquatic invertebrate toxicity study was performed. Five daphnids were exposed to each concentration, with four replicates per concentration. The measured concentrations tested were below the limit of quantification, 1.20, 3.07, 8.05, and 18.44 mg/L. Daphnids at the 8.05 mg/L concentration showed less activity than at lower concentrations or controls. The NOEC was determined to be 3.07 mg/L based on active ingredient. The EC50 was determined to be 9.06 mg/L.