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EC number: 849-166-3
CAS number: 83841-00-3
The test is considered valid as in the control no daphnids showed immobilization (should be ≤10%) or other signs of disease or stress (e.g. discoloration or unusual behavior such as trapping at the water surface). Furthermore, the dissolved oxygen concentration at the end of the test was 8.8 mg/L in the control and test vessels (should be ≥3 mg/L).
The acute toxicity of the test item to young (≤24 hours old) water flea (Daphnia magna) was determined under GLP in a 48 hour static test according to OECD TG 202, adopted 2004. The validity criteria were met. On the basis of the pre-test results, a limit test was performed in accordance with the test guideline to demonstrate that the test item has no toxic effect on the test organisms up to and including the nominal concentration of 100 mg/L during an exposure period of 48 hours. A control was tested in parallel. A static test design was applied. For each treatment, 20 daphnids were used divided into four replicates of five daphnids each. The immobility of the daphnids was determined by visual inspection after 24 and 48 hours of exposure. Observations were also performed for other non-lethal effects (e.g. abnormal behaviour or appearance). No remarkable observations were made concerning the appearance of the test medium. The test medium was a clear solution throughout the entire test duration. The measured concentration of the test item in the test medium was 102 mg/L at the start of the test and 110 mg/L at the end, 102% and 110% of nominal, respectively. No adverse effects were observed in the test animals.
48-hour EC50 >100 mg/L, water flea (Daphnia magna)
The acute toxicity of the substance to the freshwater invertebrate water flea (Daphnia magna) was investigated under GLP to OECD TG 202. The substance had no adverse effects on the test organisms over the exposure period of 48 hours, and the EC50 was greater than 100 mg/L.
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