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EC number: 849-166-3
CAS number: 83841-00-3
The ready biodegradability of the test item was investigated under GLP to the manometric respirometry test carried out according to the OECD TG 301 F (1992). The test concentration was 88 mg/L in a manometric respirometry test using domestic aerobic activated sewage sludge incubated at 22 °C in the dark over 28 days under continuous stirring. The biochemical oxygen demand (BOD) was measured daily and the percentage degradation was calculated from the theoretical oxygen demand (ThOD) after correcting for the BOD of inoculum controls. The average percent biodegradation of the test item in the test media was found to be 156% (SD = 5%) based on ThODNH3 and 87% (SD = 3%) based on ThODNO3 after 28 days of incubation. As these results are indicative of nitrification, degradation of the test item was evaluated based on ThODNO3. Considering total nitrification, the 10-day window started on Day 13, and an average biodegradation of 60% (SD = 1%) was observed at the end of the window (Day 23). Since the threshold value of 60 % was reached in a 10-day-window within the 28-d period, the test item was readily biodegradable under the test conditions. As the percent degradation of the test item was ≥60% based on complete nitrification, corrections, using measured Nitrite-N and nitrate-N concentrations, to account for nitrification were not applied. The test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration. In the procedural controls, the reference substance sodium benzoate was degraded by an average of 85% by exposure day 14, and reached an average biodegradation of 91% by the end of the test (day 28), thus confirming suitability of the activated sludge.
Readily biodegradable, OECD TG 301F, Simon 2020
The substance was found to be readily biodegradable in a manometric respirometry test performed to OECD TG 301F (1992). Degradation of the test item was evaluated based on ThODNO3. The threshold value of 60% degradation was reached in a 10-day-window within the 28-day period, and the test item was readily biodegradable under the test conditions. No corrections using measured Nitrite-N and nitrate-N concentrations were applied. The test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration. The reference substance sodium benzoate was degraded by an average of 85% by exposure day 14, and reached an average biodegradation of 91% by the end of the test, confirming suitability of the activated sludge.
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