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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure following national standard guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German standard test guideline, DIN 38 412 L15, 1982. Comparable to OECD 203.
GLP compliance:
no

Test material

Constituent 1
Details on test material:
- Name of test material (as cited in study report): Dimethylcyclohexylamine- Purity: 99.9 %- Lot/batch No.: II/87
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):Not applicable

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
No data provided

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM- Common name: Golden orfe- Strain: : Leuciscus idus L. (golden variety)- Source: Fish farm Paul Eggers, 2354 Hohenwestedt, Germany- Length at study initiation (length definition, mean, range and SD): 5.3 cm (range 4.7 - 5.7 cm)- Weight at study initiation (mean and range, SD): 3.7 g (range 2.1 - 4.9 g)- Feeding during test: no - Frequency: Withdrawal of food before exposure: 1 dayACCLIMATION- Acclimation period: 4 weeks - Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through systemWater temperature: 20 °C Duration of housing: about 4 weeksAcclimation to test water and test temperature: 3 days- Type and amount of food: : Growing feed F/B 50, - Feeding frequency: ad libitum.- Health during acclimation (any mortality observed):Mortality during the last 2 weeks of housing: 0.0 %Mortality during the acclimation period: 0 %Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
2.5 mmol/L
Test temperature:
19 - 20 °C
pH:
about 8.0
Dissolved oxygen:
8.5 ± 0.5 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
nominal: 10.0, 21.5, 46.4, 100 mg/L. Additionally: 100 mg/L pH adjusted
Details on test conditions:
TEST SYSTEM- Test vessel: - Type (delete if not applicable): aquaria open - Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L - Aeration: aeration with oil-free air- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 1- Biomass loading rate: 3.7 g fish /LTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11- Ca/Mg ratio: 4 : 1- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hoursOTHER TEST CONDITIONS- Adjustment of pH: one test item concentration of 100 mg/L was pH adjusted- Photoperiod: 16:8 hour light:dark- Aeration: slight EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptomsTEST CONCENTRATIONS- Spacing factor for test concentrations: 2.2
Reference substance (positive control):
yes
Remarks:
Chloroacetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
21.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
21.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
31.58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralised
Basis for effect:
mortality (fish)
Remarks on result:
other: geometrical mean
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
46.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
not neutralized
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Neutralized, after pH adjustment
Basis for effect:
mortality (fish)
Remarks on result:
other: after pH-adjustment
Details on results:
There were no abnormalities.
Results with reference substance (positive control):
- Results with reference substance valid? Yes- LC50: 26 mg/L after 48 h
Reported statistics and error estimates:
Observation of the results.

Any other information on results incl. tables

Sublethal observations / clinical signs:
Effects may be due to the pH of the substance: no effects were observed after 96 -h when exposed to a neutralized sample (100 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Groups of ten Golden orfe (Leuciscus idus L., Golden variety) were exposed under static conditions to DMCHA, for a period of 96 -hours.  The 96 -hour LC50 was observed to be > 21.5 mg/L and < 46.4 mg/L, the corresponding no-observed effect concentration (NOEC) was 21.5 mg/L.  If the results are based on the pH adjusted medium the 96 hour LC would be >100 mg/L and the corresponding NOEC would be 100 mg/L.
Executive summary:

Groups of ten Golden orfe (Leuciscus idus L., Golden variety) were exposed under static conditions to cyclohexyldimethylamine (DMCHA), for a period of 96 -hours according to the German Standard Test Guideline, DIN 38 412 L15, 1982 which is comparable to OECD 203. Nominal exposure concentrations were control, 0.0, 21.5, 46.4, 100 mg/L, additionally: 100 mg/L pH adjusted. No analytical verification was performed therefore the results of the study were based on nominal exposure concentrations. After 96 hours 100% mortality was observed in the 46.4 and 100 (no pH adjustment) mg/L treatments, there was no mortality in any of the other treatments or control.

The toxic effect seen in the 46.4 and 100 mg/L (no pH adjustment) was due to the high pH levels in the test media. There was no mortality in the 100 mg/L test medium when the pH was adjusted to 7.8 prior to the addition of fish.

The 96 -hour LC50 was 31.6 mg/L (geometric mean), the corresponding no-observed effect concentration (NOEC) was 21.5 mg/L. 

If the results are based on the pH adjusted medium the 96 hour LC would be >100 mg/L and the corresponding NOEC would be 100 mg/L.