Vinylphosphonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April - July 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted: 04. April 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC Directive 79/831 Annex V

Version / remarks:

Methods for the Determination of Ecotoxicity 5.1.2 Acute Toxicity for Daphnia Stand Mai 1984

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Homogenization with Ultra-Turrax and ultrasonic bath

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain/clone: STRAUS
- Source: Stock-culture of the Department of Toxicology (Hoechst AG)
- Age of parental stock: max 42 days
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21.3 - 22.0

pH:

8.2 - 8.3

Dissolved oxygen:

8.2 - 9.4 mg/L

Nominal and measured concentrations:

Nominal concentrations: 560, 1000 mg/L
Measured concentrations: 1003.2 mg/L (only the control and the highest concentration were analytically determined)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Material, size, fill volume: glass, hight 110mm diameter 70mm, 200 mL
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Elendt Medium with desalinated water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 0.18 - 0.28 ml 10 molar NaOH
- Light: fluorescent tubes in a day/night cycle, with each period lasting 12 hours

EFFECT PARAMETERS MEASURED :
After 24 and 48 hours: inspection for immobility and recording of water parameters

RANGE-FINDING STUDY
yes, but no details available

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Sampling date	1992-07-09		1992-07-11		Geometric mean measured concentration [mg/L]
Nominal concentration	Meas. conc. [mg/L]	%	Meas. conc. [mg/L]	%
Control	<LOQ		<LOQ
1000 mg/L	999.3	99.93	1007	100.7	1003.15

Meas. conc. = Measured concentration of the test item, enrichment and dilution factor taken into account

% = Percent of nominal concentration of test item

LOQ = Limit of Quantification (0.1 mg/L test item)

Validity criteria fulfilled:

yes

Conclusions:

The 48-h acute toxicity test to Daphnia magna revealed a nominal EC0 value of 1000 mg/L and an EC100 and EC50 value of >1000 mg/L after 48 hours.

Executive summary:

The 48-h acute toxicity of the test item to Daphnia magna was studied under static conditions according to OECD 202 (1984) and EEC Directive 79/831 (1984). Daphnids were exposed to the test item at nominal concentrations of 560 and 1000 mg/L. An analytical monitoring was performed. To produce the stock solution the test substance was weighed into water and homogenized with Ultra-Turrax and ultrasonic bath. During the study the dissolved oxygen, pH, water temperature and the immobilization rate were measured. Based on the number of immobile daphnids the EC values were determined. The EC0 value was 1000 mg/L (measured) and the EC100 was > 1000 mg/L (measured). The EC50 value was determined to be >1000 mg/L.

Description of key information

The 48-h acute toxicity test to Daphnia magna revealed a nominal EC0 value of 1000 mg/L and an EC100 and EC50 value of >1000 mg/L after 48 hours.

Additional information

The 48-h acute toxicity of the test item to Daphnia magna was studied under static conditions according to OECD 202 (1984) and EEC Directive 79/831 (1984). Daphnids were exposed to the test item at nominal concentrations of 560 and 1000 mg/L. An analytical monitoring was performed. To produce the stock solution the test substance was weighed into water and homogenized with Ultra-Turrax and ultrasonic bath. During the study the dissolved oxygen, pH, water temperature and the immobilization rate were measured. Based on the number of immobile daphnids the EC values were determined. The EC0 value was 1000 mg/L (measured) and the EC100 was > 1000 mg/L (measured). The EC50 value was determined to be >1000 mg/L.