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EC number: 276-851-9
CAS number: 72797-41-2
This in vitro study was performed to assess the corrosive potential of
1574 ACIDE CRIST by means of the Human Skin Model Test with EpiDerm™
The test item did not reduce MTT (test for direct MTT reduction), and it
did not change colour when mixed with deionised water (test for colour
interference). Consequently, additional tests with freeze-killed or
viable tissues to determine correction factors for calculating the true
viability in the main experiment were not necessary.
Independent duplicate tissues of EpiDerm™ were exposed to either the
test item, the negative control (deionised water) or the positive
control (8.0 N KOH) for 3 minutes and 1 hour, respectively.
After exposure to the negative control the absorbance values met the
required acceptability criterion of a mean OD570 ≥ 0.8 and ≤ 2.8 for
both treatment intervals thereby confirming the acceptable quality of
Exposure to the positive control induced a decrease in the relative
absorbance as compared to the negative control, both for the 3 minutes
exposure period and for the 1 hour exposure period. The 1 hour exposure
caused a decrease of the cell viability < 15% of the negative control.
The CV in the range 20 – 100% viability between the tissue replicates is
≤ 30%, thus the validity of the test system and the specific batch of
the tissue models is confirmed.
After exposure of the tissues to the test item the relative absorbance
value did not decrease (103.1%) after 3 minutes exposure. After 1 hour
exposure the relative absorbance value was reduced to 90.6%. Both values
did not affect the threshold for corrosivity which is defined to be 50%
after the 3 minutes exposure and 15% after the 1 hour exposure.
Therefore, the test item is not considered to be corrosive.
In conclusion, it can be stated that in this study and under the
reported experimental conditions, the test item 1574 ACIDE CRIST is non
corrosive to skin according to EU CLP and UN GHS
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