1,3,5-tris(2,3-dibromopropyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: The Consumer Product Safety Commission of the U.S.A. in The Code of Federal Regulations, Title 16, Section 1500.42

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-tris(2,3-dibromopropyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione

EC Number:

257-913-4

EC Name:

1,3,5-tris(2,3-dibromopropyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione

Cas Number:

52434-90-9

Molecular formula:

C12H15Br6N3O3

IUPAC Name:

tris(2,3-dibromopropyl)-1,3,5-triazinane-2,4,6-trione

impurity 1

Reference substance name:

unknown impurities

IUPAC Name:

unknown impurities

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Sample code n°: 507-43

Test animals / tissue source

Species:

rabbit

Strain:

other: Albino rabbit

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes

Amount / concentration applied:

79 mg

Observation period (in vivo):

14 days

Number of animals or in vitro replicates:

6 animals

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

1

Reversibility:

fully reversible within: 1 day

Results 2

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

0.72

Max. score:

1

Reversibility:

fully reversible within: 7 days

Results 3

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Results 4

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Irritant / corrosive response data:

The numerical scores awarded to the ocular reactions elicited by test sample 507-43 are given in section "Any other information on results incl. tables".
None of the animals gave a positive reaction. Mild conjunctival reactions (redness) were observed in all six animals.

Any other information on results incl. tables

The numerical scores awarded to the ocular reactions elicited by Sample 507-43 are presented in Table 1 below:  

 

Table 1

Animal No.	Region of eye	Day						Positive (+) or Negative (-) Result
		1	2	3	4	7	14
1	Cornea	0	0	0	0	0	0	-
	Iris	0	0	0	0	0	0
	Conjunctivae redness	0	1	1	1	1	0
	Conjunctivae chemosis	0	0	0	0	0	0
2	Cornea	0	0	0	0	0	0	-
	Iris	0	0	0	0	0	0
	Conjunctivae redness	1	1	1	1	1	0
	Conjunctivae chemosis	1	0	0	0	0	0
3	Cornea	0	0	0	0	0	0	-
	Iris	0	0	0	0	0	0
	Conjunctivae redness	1	1	1	0	0	0
	Conjunctivae chemosis	0	0	0	0	0	0
4	Cornea	0	0	0	0	0	0	-
	Iris	0	0	0	0	0	0
	Conjunctivae redness	1	1	1	0	0	0
	Conjunctivae chemosis	0	0	0	0	0	0
5	Cornea	0	0	0	0	0	0	-
	Iris	0	0	0	0	0	0
	Conjunctivae redness	1	0	0	0	0	0
	Conjunctivae chemosis	0	0	0	0	0	0
6	Cornea	0	0	0	0	0	0	-
	Iris	0	0	0	0	0	0
	Conjunctivae redness	1	0	0	0	0	0
	Conjunctivae chemosis	0	0	0	0	0	0

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Sample 507-43 gave a negative result for eye irritation, according to the definition given in the Code of Federal Regulations of the U.S.A.

Executive summary:

The procedure employed was that prescribed by The Consumer Product Safety Commission of the U.S.A. in The Code of Federal Regulations, Title 16, Section 1500.42, a transcript of which is attached as an Appendix to the full study report.

For the test, 79 mg of sample 507-43 was instilled into one eye of each six animals, while the untreated eye was used as concurrent control. 

The numerical scores awarded to the ocular reactions elicited by 507-43 are given in Table 1: none of the animals gave a positive reaction and only mild conjunctival reactions were observed in all six animals.

Sample 507-43 gave a negative test for eye irritation, according to the definition given in the Code of Federal Regulations of the U.S.A.