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EC number: 257-913-4
CAS number: 52434-90-9
The effects of AP 729 were tested according to OECD Test Guideline 201 on the growth of freshwater unicellular green alga Raphidocelis subcapitata (formerly Pseudokirchneriella subcapitata) for 72 hours.
The algae were exposed to the test item at the test concentrations of 50, 80, 128, 204.8 and 327.7 mg/L (factor of 1.6) along with a negative control and vehicle control. Six replicates were maintained for the negative control and the vehicle control, and three replicates for the test concentrations. The cell growth was measured at 24, 48 and 72 hours after the initiation of the test using a haemocytometer (microscopic observation).The concentration-effect relationship was determined using two factors, namely growth rate and yield at the end of the test (72 hours). The inhibition of growth rate was 0, 10.11, 29.30, 58.54 and 92.01% and the inhibition of yield 0.0, 36.30, 73.28, 93.50 and 99.48% at the nominal concentrations 50, 80, 128, 204.8 and 327.7 mg/L respectively, when compared to the vehicle control. The test item concentration analysis in all test concentrations showed that the mean percent recovery with the nominal concentration was from 94.592 to 107.275% at the start of the test (RSD was 0.085 to 0.491%) and 81.644 to 95.151% at the end of the test (72 hours) (RSD was 0.262 to 0.777%) indicating that the results were within the acceptable limit (80 to 120% of the nominal concentration with an RSD of < 20%).Observed cells were found morphologically normal in all the tested concentrations.
The endpoint values for growth rate and yield at 72 hours, based on nominal concentration, are presented below in Table 1:
At 72 hours
EC values (mg/L)
The algae were exposed to the test item at the test concentrations of 50, 80, 128, 204.8 and 327.7 mg/L along with a negative control and vehicle control. Six replicates were maintained for the negative control and the vehicle control, and three replicates for the test concentrations.
The concentration-effect relationship was determined using two factors, namely growth rate and yield at the end of the test.
The validity criteria of the test were fulfilled.
The following EC50 values were found: ErC50 = 170.37 mg/L, EyC50 = 95.183 mg/L.
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