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EC number: 292-222-1 | CAS number: 90583-23-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Jan -02 Feb 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.68 g test substance were dissolved in 1L VE-water - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: CHODAT 86.81 SAG
- Source (laboratory, culture collection): Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age of inoculum (at test initiation): 3 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- not applicable
- Test temperature:
- 22.1-22.3 °C
- pH:
- Begin: 8.2-8.3
End: 7.8-9.4 - Nominal and measured concentrations:
- Nominal: 0, 0.6, 1.25, 2.5, 5, 10 and 20 mg/L (concentration of active ingredient)
Measured: 0.12, 0.65, 1.2, 2.3, 4.6, 9.7 and 20 mg/L (0 hours, concentration of active ingredient)
Measured: 0.0, 0.61, 0.87, 1.8, 3.4, 12 and 17 mg/L (72 hours, concentration of active ingredient) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Aeration: sterile aeration
- Renewal rate of test solution (frequency/flow rate): none
- Initial cells density: 20,000 cells/mL
- Control end cells density: 1,040,000 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24 hours
- Light intensity and quality: 8000 Lux, white
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: with spectrophotometer at at 685 nm
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Reported statistics and error estimates:
- Probit analysis according to Cavalli-Sforza (1972)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- growth rate
- Remarks on result:
- other: Source, key, CAS 85586-07-8, 1995
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- growth rate
- Remarks on result:
- other: Source, key, CAS 85586-07-8, 1995
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- growth rate
- Remarks on result:
- other: Source, key, CAS 85586-07-8, 1995
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- biomass
- Remarks on result:
- other: Source, key, CAS 85586-07-8, 1995
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: a-WAS
- Basis for effect:
- biomass
- Remarks on result:
- other: Source, key, CAS 85586-07-8, 1995
Referenceopen allclose all
Description of key information
EC50 (72h, growth rate) = > 20 mg/L (Desmodesmus subspicatus, nominal concentration, read across, EU Method C.3)
EC10 (72h, growth rate) = 5.4 (Desmodesmus subspicatus, nominal concentration, read across, EU Method C.3)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 20 mg/L
- EC10 or NOEC for freshwater algae:
- 5.4 mg/L
Additional information
Since no toxicity tests with algae are available for C12-14AS Mg (CAS 90583-23-6), read-across to other structurally related category members was conducted and corroborates the findings of the study mentioned above. Read-across data from a similar mixture C12-14AS Na (CAS 85586-07-8) were used. Toxicity of alkyl sulfates is dependent on the carbon chain length and the influence of the counter ion can be neglected.
The toxicity of sulfuric acid, mono-C12-14 alkyl esters, sodium salts (CAS 85586-07-8) to aquatic algae was investigated in the GLP-compliant study with Desmodesmus subspicatus according to EU Method C.3 (Elementis 1995). Algae were exposed to six test concentrations up to 20 mg/L (nominal concentration corresponding to 17 mg/L of measured concentration). The study resulted in the 72-hour EC50 value for growth rate of above 20 mg a.i./L (nominal concentration). The EC10 (72 h) for growth rate was 5.4 mg a.i./L (nominal concentration).
Due to structural and property similarity with the tested category member, the 72-hour EC50 value of >20 mg/L and EC10 (72h) of 5.4 mg/L (both for growth rate) is considered as reliable for C12-14 Mg (CAS 90583-23-6) for freshwater algae.
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