Sulfuric acid, mono-C12-14-alkyl esters, magnesium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 Mar - 03 Mar 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EG-guideline EG/92/69/EWG

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 0.6, 1.3, 2.5, 5.0 and 10.0 mg/L

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: waterflea
- Strain: Clone 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: Daphnids held in 1 L beakers with M4 Medium (Elendt), every 2-3 days juveniles are removed and water changed, every 4 weeks a generation change is induced by continuing the breeding with juveniles only
- Feeding during test: no

ACCLIMATION (in-house culture conditions)
- Type and amount of food: Scenedesmus subspicatus ad libidum
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

no data

Test temperature:

20 ± 1 °C

pH:

Test end: 7.8 - 8.3

Dissolved oxygen:

Test end: 7.2 - 8.2 mg/L

Nominal and measured concentrations:

Nominal: 0, 0.6, 1.3, 2.5, 5.0 and 10.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 mL hourglasses
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility, daily

Reference substance (positive control):

no

Remarks:

Reference substances potassium dichromate was tested in a separate study

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: C.L.: 2.5 - 5.0 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Potassium dichromate was tested as reference substance in a separate test (11 Jan 1995)
- Results with reference substance valid? yes
- Mortality: 100% immobilised at 2 mg/L; 35% immobilised at 1 mg/L

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Refer to the Category Approach Justification document provided in IUCLID6 Section 13

Reason / purpose for cross-reference:

read-across source

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Source, key, CAS 85586-07-8, 1995

Description of key information

48h EC50 (Daphnia magna, mobility) = 4.7 a.i.m/L (nominal concentration, EG/92/69/EWG)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

4.7 mg/L

Additional information

Since no reliable chronic tests involving aquatic invertebrates are available for C12-14AS Mg (CAS 90583-23-6), a read-across to structurally related category members was conducted in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5. Read-across data from a similar substance, C12-14 AS Na (CAS 85586-07-8) were used. Toxicity of alkyl sulfates is dependent on the carbon chain length and the influence of the counter ion can be neglected.

One short term toxicity test with aquatic invertebrates is available for C12-14 AS Na (CAS 85586-07-8, Elementis, 1995). This GLP test was preformed according to the guideline EG/92/69/EWG under static conditions. Young daphnids were exposed to five test concentrations up to 10 mg a.i./L. The EC50 value after 48h was determined to be 4.7 mg a.i./L. Measured concentrations were in agreement with the nominal concentrations.